乌药提取物对胃实寒模型大鼠cAMP, cGMP, GAS, MTL水平的影响

目的:观察乌药对胃实寒模型大鼠血浆中环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、胃泌素(GAS)、胃动素(MTL)的影响。方法:模型组大鼠采用寒冷刺激法造模3 d制备胃实寒模型,按前后体重差随机分成8组,分别为模型对照组、吗丁啉组、红花水煎液组、乌药水煎液组、乌药挥发油组、乌药醇提液组、乌药水提乙醚萃取物组、乌药醇提乙醚萃取物组,乌药各提取物给药剂量均为生药1.64 g.kg-1,第4 d造模后10 min,给药组ig相应药液10 mL.kg-1,空白对照组及模型对照组给予等体积蒸馏水,1 h后进行第2次给药,以酚红法测定胃排空率。采用酶联免疫法测定大鼠血浆中cAMP,cGMP,MTL和血清GAS的含量。结果:与正常组比较,模型组胃排空率显著降低(P<0.05),cGMP,cAMP/cGMP降低(P<0.05),cAMP,GAS,MTL值无显著差异。与模型组比较,乌药水煎液、乌药挥发油、乌药醇提乙醚萃取物能明显抑制胃排空率(P<0.05或P<0.01);乌药水煎液和乌药挥发油能明显升高cAMP含量(P<0.01),乌药水煎液、乌药挥发油、乌药醇提液、能够明显降低cGMP含量(P<0.01),乌药水煎液、乌药挥发油、乌药醇提乙醚萃取物能明显升高cAMP/cGMP(P<0.01)。结论:乌药水煎液、挥发油和醇提乙醚萃取物组能够明显抑制胃排空,明显升高cAMP/cGMP。

Effect of Extraction of Linderae Radix on cAMP cGMP GAS MTL Level in Rat Stomach Cold Models

Objective: To observe the influence of Linderae Radix on cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate(cGMP), gastrin (GAS), motilin (MTL) of plasma in rat stomach cold models. Methods: The rats of model group were prepared by using cold stimulation method for three days. According to the difference of weight between before and after modelling, the rats were randomly divided into eight groups: model group, domperidone group, water decoction of Safflower group, the water decoction group, the volatile medicine group, the medicine ethanol-extraction liquid group, the potions mention ether extract group, the medicine ethanol-extraction ether extract group. At 10 minutes after modeling of the fourth day, treatment group was given corresponding solution 1 mL·kg^-1, normal group and model group were given distilled water e, the second administration was carried out after one hour, the gastric emptying rate was identified through Phenol red method. the content of plasma cAMP, cGMP, MTL and serum GAS was examined. Result: Compared with the normal group, the gastric emptying rate of model group significantly reduced(P<0.05),levels of cGMP,cAMP / cGMP were reduced(P<0.05),and levels of CAMP,GAS,MTL had no significant difference.Compared with model group, treatment goups like the medicine decoction, volatile oil and uriah medicine ethanol-extraction ether extract of Linderae Radix significantly suppressed gastric emptying rate(P<0.05, P<0.01). The medicine decoction and volatile oil of Linderae Radix could obviously increase the content of cAMP(P<0.01); the medicine decoction, volatile oil, lindera root alcohol extract and alcohol extraction ether extract of Linderae Radix could obviously reduce the content of cGMP(P<0.01); the medicine decoction and volatile oil of Linderae Radix could significantly increased the ratio of cAMP/cGMP(P<0.01). Conclusion: The medicine decoction, volatile oil and uriah medicine ethanol-extraction ether extract of Radix Linderae can inhibit significantly gastric emptying, and can markedly elevate cAMP/cGMP.