双波长HPLC同时测定延胡索中3种生物碱的含量

目的:建立同时测定延胡索中巴马汀、脱氢紫堇碱和延胡索乙素的HPLC方法.方法:采用C18柱(4.6 mm×250nm,5 μm),以乙腈(A)-0.1％磷酸(加三乙胺调节pH 6.0,B)进行梯度洗脱(0～ 20 min,25％A;20～30 min,25％～55％ A;30 ～45 min,55％A;45 ～ 50 min,55％～25％A;50 ～55 min,25％A);检测波长为270,280 nm,柱温30℃.结果:巴马汀在2.12×10-2～1.59 μg呈良好的线性关系(r=0.999 9);脱氢紫堇碱在4.10 e 10-2～ 3.07 μg呈良好的线性关系(r=0.999 9);延胡索乙素在2.10×10-2～1.57 μg呈良好的线性关系(r =0.999 9);加样回收率分别为100.10％,100.03％,97.47％.结论:该方法准确、稳定、分离效果好,可用于测定延胡索药材中巴马汀、脱氢紫堇碱和延胡索乙素的含量.

Determination of Three Active Components in Rhizoma Corydalis by Dual Wavelength HPLC

Objective:To develop a new method for determination of palmatine hydrochloride,dehydrocorydaline nitrate and tetrahydropalmatine in Rhizoma Corydalis by HPLC.Method:The HPLC was adopted performing on a C18column(4.6 mm×250 nm,5 μm) with a mobile phase of acetonitrile(A)-0.1%phosphoric acid(B).The gradient elution program was as follows: 0-20 min,25%A;20-30 min,25%-55%A;30-45 min,55%A;45-50 min,55%-25%A;50-55 min,25%A;The detection wavelength was set at 270 nm and 280 nm.The column temperature was at 30 ℃.Result:Palmatine hydrochloride was linear in the range of 2.12×10-2-1.59 μg(r=0.999 9);dehydrocorydaline nitrate was linear in the range of 4.10×10-2-3.07 μg(r=0.999 9);tetrahydropalmatine was linear in the range of 2.10×10-2-1.57 μg(r=0.999 9).The average recovery was 100.10%,100.03% and 97.47%.Conclusion:The method was accurate and had satisfactory efficacy;it can determine palmatine hydrochloride,dehydrocorydaline nitrate and tetrahydropalmatine in Rhizoma Corydalis.