| RP-HPLC同时测定不同根龄虎杖根中虎杖苷和白藜芦醇 |
| |
| 引用本文： | 梁辉,邹盛勤.RP-HPLC同时测定不同根龄虎杖根中虎杖苷和白藜芦醇[J].中国实验方剂学杂志,2011,17(23):62-64. |
| |
| 作者姓名： | 梁辉 邹盛勤 |
| |
| 作者单位： | 宜春学院化学与生物工程学院,江西省天然药物活性成分研究重点实验室,江西宜春336000 |
| |
| 摘 要： | 目的:建立反相高效液相色谱法同时测定不同根龄虎杖根中虎杖苷和白藜芦醇含量。方法:采用Kromasil C18(4.6 mm×250 mm,5μm)色谱柱,流动相甲醇-1.0%磷酸水溶液(50∶50),流速0.8 mL.min-1,检测波长319 nm,柱温为30℃。结果:虎杖苷和白藜芦醇的进样量分别在0.716～7.160μg(r=0.999 6)和0.740～7.400μg(r=0.999 8)与峰面积呈良好的线性关系;平均回收率(n=6)分别为99.7%,98.5%。结论:本方法快速简便,重复性好,专属性强,可作为虎杖根的质量控制方法。
|
| 关 键 词： | 虎杖苷 白藜芦醇 根龄 虎杖 反相高效液相色谱 |
| 收稿时间： | 4/5/2011 12:00:00 AM |
Simultaneous Determination of Polygonin and Resveratrol in Different Age Root of Polygonum cuspidatum by RP-HPLC |
| |
| LIANG Hui and ZOU Sheng-qin.Simultaneous Determination of Polygonin and Resveratrol in Different Age Root of Polygonum cuspidatum by RP-HPLC[J].China Journal of Experimental Traditional Medical Formulae,2011,17(23):62-64. |
| |
| Authors: | LIANG Hui and ZOU Sheng-qin |
| |
| Institution: | College of Chemistry and Bioengineering of Yichun University, Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun 336000, China;College of Chemistry and Bioengineering of Yichun University, Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun 336000, China |
| |
| Abstract: | |
| |
| Keywords: | polygonin resveratrol root age polygonum cuspidatum RP-HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
| 点击此处可从《中国实验方剂学杂志》下载免费的PDF全文 |
