| HPLC测定增生药膏中士的宁的含量 |
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| 引用本文： | 蒙麦侠,杨智峰,詹菊红,郑洁.HPLC测定增生药膏中士的宁的含量[J].中国中医药信息杂志,2011,18(11):53-54. |
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| 作者姓名： | 蒙麦侠 杨智峰 詹菊红 郑洁 |
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| 作者单位： | 1. 陕西省中医药研究院,陕西西安,710013
2. 西安交通大学,陕西西安,710049 |
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| 摘 要： | 目的建立增生药膏中士的宁的测定方法。方法采用高效液相色谱法,色谱柱为kromasil C18柱,流动相为乙腈∶0.01 mol/L庚烷磺酸钠与0.02 mol/L磷酸二氢钾等量混合溶液（加10%磷酸调节pH值至2.8）=21∶79,检测波长260 nm,柱温30℃,流速1 mL/min。结果士的宁在0.125 6～0.471 0μg范围内,进样量与峰面积的线性关系良好（r2=0.9999）,平均回收率为98.45%,RSD=0.85%。结论该方法简便快捷,重复性好,可作为增生药膏中士的宁的测定方法。
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| 关 键 词： | 士的宁 增生药膏 高效液相色谱法 含量测定 |
Determination of Strychnine in Zengsheng Yaogao by HPLC |
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| MENG Mai-xia,YANG Zhi-feng,ZHAN Ju-hong,ZHENG Jie.Determination of Strychnine in Zengsheng Yaogao by HPLC[J].Chinese Journal of Information on Traditional Chinese Medicine,2011,18(11):53-54. |
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| Authors: | MENG Mai-xia YANG Zhi-feng ZHAN Ju-hong ZHENG Jie |
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| Institution: | MENG Mai-xia1,YANG Zhi-feng1,ZHAN Ju-hong2,ZHENG Jie1(1.Academy of Traditional Chinese Medicine and Materia Medica of Shannxi Province,Xi'an 710013,China,2.Xi'an Jiaotong University,Xi'an 710049,China) |
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| Abstract: | Objective To establish the method for determining the content of strychnine in Zengsheng Yaogao by HPLC.Methods A HPLC method was carried on kromasil C18 column,with acetonitril：equal volume of 0.01 mol/L sodium heptanesulfonate and 0.02 mol/L potassium dihydrogen phosphate sodium（adjusted pH to 2.8 by 10% phosphoric acid）=21∶79 as mobile phase.The UV detection wavelength was 260 nm and the flow rate was 1.0 mL/min.Results The calibration curve of strychnine showed good linearity（r2=0.999 9） within the range of 0.125 6-0.471 0 ？g and the average recovery was 98.45%,RSD was 0.85%.Conclusion The method is simple,rapid and with good reproducibility for the determination of strychnine in Zengsheng Yaogao. |
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| Keywords: | strychnine Zengsheng Yaogao HPLC content determination |
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