反相高效液相色谱法同时测定人血浆中替米沙坦、氢氯噻嗪含量

 目的建立可同时检测人血浆中替米沙坦、氢氯噻嗪含量的反相高效液相色谱方法。方法色谱条件:色谱柱为Zorbax80A Extend-C₁₈(4.6 mm×150 mm,5μm);流动相为乙腈-四丁基氢氧化铵磷酸盐缓冲液(24∶76);流速:1.0 mL·min^-1;检测波长:230 nm(替米沙坦),272 nm(氢氯噻嗪);柱温:20℃。结果本法替米沙坦的线性范围为31.8～1 591.2μg·L^-1,r=0.999 4,方法回收率为98.68%,RSD=3.09%,最低检测质量浓度为20μg·L^-1。氢氯噻嗪的线性范围为30～240μg·L^-1,r=0.999 1,方法回收率为100.32%,RSD=5.11%,最低检测质量浓度为20μg·L^-1。结论本法可以同时测定血浆中替米沙坦和氢氯噻嗪的浓度,具有样品处理简单、方法稳定、有较好的实用性,易于开展药动学及药效学研究。

Simultaneous Determination of Telmisartan and Hydrochlorothiazide in Human Plasma by RP-HPLC

OBJECTIVE To establish a method for simultaneous determination of telmisartan and hydrochlorothiazide in human plasma.METHODS The concentrations of telmisartan and HCTZ were analysed on a Zorbax 80A Extend-C₁₈(4.6 mm×150 mm,5 μm)column with a mobile phase consisting of tetrabutylammonium hydroxide phosphate buffer solution(pH 2.26)-acetonitrile(76∶24) at a flow rate of 1 mL·min^-1,the wavelengthes were 230 and 272 nm,respectively.RESULTS The calibration curve of TMST was linear with the range of 31.8～1 591.2 μg·L^-1.The minimum concentration was 20 μg·L^-1.The calibration curve of hydrochlorothiazide was linear with the range of 30~240 μg·L^-1.The minimum concentration was 20 μg·L^-1.CONCLUSION It is a rapid,reproducible method.Clinical application demonstrated that this method was practical and it can be completely used in the study of pharmacokinetics and pharmacodynamics.