艾灸对三阴性乳腺癌移植瘤小鼠生存期的影响及机制研究

目的:观察艾灸对三阴性乳腺癌(TNBC)移植瘤小鼠生存期的影响,并基于对血清外泌体的分析探讨艾灸的干预机制。方法:采用TNBC细胞系皮下移植构建小鼠移植瘤模型,随机分为对照组与实验组; 分析小鼠生存期,绘制生存曲线,并检测血清外泌体。结果:实验组小鼠最长生存期达到53 d,对照组小鼠最长生存期48 d,两组比较差异有统计学意义(P<0.05); 通过透射电镜观察提取物形态,纳米颗粒跟踪分析仪测定粒径分布及浓度,Western blot检测外泌体表面标记蛋白,最终确定该提取物为血清外泌体。两组小鼠血清提取物均有CD63、TSG101表达。两组小鼠外泌体浓度分别为(6.19×10⁹)个/ml、(2.34×10⁹)个/ml,两组比较差异有统计学意义(P<0.01)。结论:艾灸延长三阴性乳腺癌荷瘤小鼠生存期的机制可能与促进相关细胞释放外泌体有关。

Effect and mechanism of moxibustion on survival of triple negative breast cancer transplanted tumor mice

Objective:To observe the effect of moxibustion on the survival of triple negative breast cancer(TNBC)transplanted tumor mice,and explore the intervention mechanism of moxibustion based on the analysis of serum exosomes.Methods:The mouse tumor model was established by subcutaneous transplantation of TNBC cell line,and was randomly divided into control group and experimental group.The survival time of mice was analyzed,the survival curve was drawn,and serum exosomes were detected.Results:The longest survival time of mice in the experimental group was 53 days,and the longest survival time of mice in the control group was 48 days,the difference was statistically significant(P<0.05).The morphology of the extract was observed by transmission electron microscopy,particle size distribution and concentration were determined by nano-particle tracking analyzer,and labeled proteins on exosome surface were detected by Western blot.Finally,the extract was confirmed to be serum exosome.CD63 and TSG101 were expressed in serum extracts of both groups.The exosome concentration of mice in the two groups was(6.19×10⁹)/ml and(2.34×10⁹)/ml,with statistically significant difference between the two groups(P<0.01).Conclusion:The mechanism of moxibustion prolonging the survival of mice with triple negative breast cancer may be related to promoting the release of exosomes from related cells.