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复方黄芪口服液中总皂苷含量测定
引用本文:杨洪元,周小斌,王裕盛.复方黄芪口服液中总皂苷含量测定[J].亚太传统医药,2013(12):26-27.
作者姓名:杨洪元  周小斌  王裕盛
作者单位:[1]南京中医药大学附属常州市中医医院药剂科,江苏常州213003 [2]江苏聚荣制药集团有限公司,江苏常州213105
摘    要:目的:建立复方黄芪口服液制剂中总皂苷的含量测定方法。方法:采用紫外分光光度法,以黄芪甲苷为对照品,在波长536nm处测定复方黄芪口服液中总皂苷的吸光度并计算其含量。结果:黄芪甲苷在0.0526-0.263μg·mL-1浓度范围内线性关系良好(r=0.9982),平均回收率为96.34%,RSD为1.57%(n=6)。结论:该方法简便、准确、重现性好,可作为复方黄芪口服液的质量控制方法。

关 键 词:紫外分光光度法  复方黄芪口服液  总皂苷  含量测定

Determination of Total Saponins in Fufanghuangqi Oral Liquid
Yang Hongyuan,Zhou Xiaobing,Wang Yusheng.Determination of Total Saponins in Fufanghuangqi Oral Liquid[J].Asia-Pacific Traditional Medicine,2013(12):26-27.
Authors:Yang Hongyuan  Zhou Xiaobing  Wang Yusheng
Institution:1. Derpartment of Pharmcy, the Traditional Medicine Hospital of Changzhou, Nangjing University Chinese Medicine, Changzhou 213003, China ; 2. Jingshu J urong Parmaceutical Group Co. Ltd,Changzhou 213018,China)
Abstract:Objective:To establish a method for the determination of the total saponins in Fufanghuangqi oral liquid. Methods: Based on the reference of Astragioside lV, the determination of total saponins by UV spectrophotometry at the wavelength of 536 nm. Restllts: AstragalosidelVshowed a good linear relationship in the range of 0. 052640. 263μg · mL -1 (r=0. 998 2), the average recovery (n= 5) was 96.34 % (RSD= 1.57 ~). Conclusion: The method was simple, relatively precise and reproducible. It can be used for the quality control of Fufanghuangqi oral liquid.
Keywords:UV Spectrophotometry  Fufanghuangqi Oral Liquid  Total Saponins  Determination
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