| HPLC-ELSD法测定益气软皮丸中黄芪甲苷含量 |
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| 引用本文： | 罗堃,;王元清,;谢瑜,;李顺祥,;严建业.HPLC-ELSD法测定益气软皮丸中黄芪甲苷含量[J].亚太传统医药,2014(21):2-4. |
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| 作者姓名： | 罗堃 ;王元清 ;谢瑜 ;李顺祥 ;严建业 |
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| 作者单位： | [1]湖南中医药大学药学院中药现代化教育厅重点实验室,湖南长沙410208; [2]中南林业科技大学生命科学与技术学院生物技术与工程实验室,湖南长沙410004 |
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| 基金项目： | 湖南省高校“中药新药创制与资源综合持续利用”科技创新团队项目（湘教通[2010]212号）; 湖南省中药活性物质筛选工程技术研究中心项目（湘科计字[2013]83号）; 湖南省“中药学”重点学科项目（湘教通[2011]76号） |
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| 摘 要： | 目的：建立高效液相色谱-蒸发光散射检测器（HPLC-ELSD）法测定益气软皮丸中黄芪甲苷含量的方法。方法：采用Agilent TC-C18色谱柱（250mm×4.6mm,5μm）,以乙腈-水（32∶68）为流动相,流速为1.0mL·min^-1,柱温为30℃,漂移管温度为100℃,载气流速为2.5mL·min^-1,进样量为20μL。结果：黄芪甲苷在1.02～10.20μg范围内线性关系良好,线性方程为Y=1.7281 X＋1.9515（r=0.9998）,平均回收率为97.59%,RSD为0.95%（n=6）。结论：该方法准确、简便、重复性好,可用于该制剂中黄芪甲苷含量的测定。
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| 关 键 词： | 益气软皮丸 黄芪甲苷 高效液相色谱-蒸发光散射检测法 含量测定 |
Determination of Astragnaloside A in Yiqi Ruanpi Pills by HPLC-ELSD |
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| Institution: | Luo Kun,Wang Yuanqing,Xie Yu,Li Shunxiang,Yan Jianye (1. Key Lab of Modernization of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine,Changsha 410208,China; 2. Lab of Biotechnology and Engineering, College of Life Science and Technology,Central South University of Forestry and Technology, Changsha 410004,China) |
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| Abstract: | Objective：To establish HPLC-ELSD method for content determination of astragaloside A in Yiqi Ruanpi Pills.Methods：The separation was performed on Agilent TC-C18（250mm×4.6mm,5μm）column with acetonitrile water（32∶68）as mobile phase.The flow rate was 1.0 mL·min^-1.The column temperature was 30℃.The temperature of drift tube for ELSD was set at 100℃,the air flow rate was 2.5mL·min^-1.Injection volume was 20μL.Results：The linear range of astragaloside A was 1.02～10.20μg,The regressive equation of tandard curve was Y=1.7281X＋1.9515（r=0.9998）.The average recovery rate was 97.59%,RSD=0.95%（n=6）.Conclusion：The method is simple and accurate,and has good reproducibility.It can be used as determination method of astragaloside IV in Yiqi Ruanpi Pills.of Yiqi Ruanpi Pill. |
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| Keywords: | Yiqi Ruanpi Pills Astragaloside A HPLC-ELSD content determination |
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