| 反相高效液相色谱法测定滇桂艾纳香中原儿茶酸的含量 |
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| 引用本文： | 谢培德,桑彤,龚秀珍.反相高效液相色谱法测定滇桂艾纳香中原儿茶酸的含量[J].中国中药杂志,2000,25(4):227-229. |
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| 作者姓名： | 谢培德 桑彤 龚秀珍 |
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| 作者单位： | 广西药品检验所!广西南宁530021 |
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| 摘 要： | 目的：建立反相高效液相色谱法测定滇桂艾纳香中原儿茶酸的方法。方法：滇桂艾纳香水提液醋酸乙酯-乙醇（4∶1）提取，薄层色谱分离，反相高效液相色谱法测定，μbondapak C18色谱柱，流动相甲醇-水-冰醋酸（19∶80∶1），检测波长260 nm。结果：进样量3.31～41.8 μg.ml－1，线性关系良好(r=0.9999)，加样回收率为98.05%，RSD=1.94%（n=6）。结论：该法为开发利用滇桂艾纳香提供依据。
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| 关 键 词： | 反相高效液相色谱法 原儿茶酸 滇桂艾纳香 |
| 收稿时间： | 4/7/1999 12:00:00 AM |
Determination of Protocatechuic Acid in Blumea riparia (Bl.) DC. by RP-HPLC |
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| XIE Pei-de;SANG Tong;GONG Xiu-zhen.Determination of Protocatechuic Acid in Blumea riparia (Bl.) DC. by RP-HPLC[J].China Journal of Chinese Materia Medica,2000,25(4):227-229. |
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| Authors: | XIE Pei-de;SANG Tong;GONG Xiu-zhen |
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| Institution: | Guangxi Provincial Institute for Drug Control, Nanning 530021, Guangxi, China. |
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| Abstract: | OBJECTIVE: To determine the content of protocatechuic acid in Blumea riparia by RP-HPLC. METHOD: mu-Bondapak C18 column was used, mobile phase consisted of methanol-water-glacial acetic acid(19:80:1) and detection was performed at UV 260 nm. RESULT: The standard curve was linear in the range of 3.31-41.8 micrograms.ml-1. The correlation coefficient was 0.9999. The average recovery rate and RSD were 98.05% and 1.94% (n = 6) respectively. CONCLUSION: The method provides scientific indexes for quality control of riparia. |
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| Keywords: | RP-HPLC protocatechuic acid Blumea riparia |
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