鸡血藤非黄酮类化学成分的研究

研究鸡血藤水提物中的酚酸酯和其他类型的化学成分。运用硅胶、D101大孔吸附树脂、Sephadex LH-20等柱色谱和反相高效液相色谱分离和纯化鸡血藤中酚酸酯类和其他类型的化合物,并运用核磁共振、高分辨质谱等谱学技术鉴定所得化合物的结构。从鸡血藤水提物的乙酸乙酯和正丁醇溶性部位分离得到酚酸、香豆素、木脂素、萜、生物碱和甾体等类化合物27个,分别鉴定为β-谷甾醇(1),阿魏酸甲酯(2),丁香脂素(3),(+)-杜仲树脂酚(4),(+)-表松脂醇(5),对羟基苯乙酮(6),树脂藤素Ⅳ(7),楝叶吴萸素B(8),水杨酸(9),反式对羟基肉桂酸(10),脱落酸(11),间苯二酚(12),C-藜芦酰乙二醇(13),对苯二酚(14),8,9-二羟基巨豆-4,6-二烯-3-酮(15),对羟基苯甲酸(16),6,9-二羟基巨豆-4,7-二烯-3-酮(17),原儿茶酸(18),原儿茶酸甲酯(19),5,7-二羟基香豆素(20),异落叶松脂素(21),烟酸(22),胡萝卜苷(23),(+)-松脂醇(24),豆甾醇(25),尿囊素(26),3,5-二甲氧基-4-羟基苯基-1-O-β-D-吡喃葡萄糖苷(27)。其中化合物2~15,19~22,24和26均系首次从该属植物中分离得到。

Study on non-flavonoids chemical constituents from Spatholobi Caulis

To study the non-flavonoids chemical constituents in water extract of Spatholobi Caulis. Some purification and analysis techniques like silica gel, D101-macroporous adsorptive resins, and Sephadex LH-20 column chromatographies as well as reversed phase high-performance liquid chromatography were used to isolate and analyze the phenolic acid esters and other type compounds from Spatholobi Caulis integrally. The structures of these compounds were identified by spectroscopic techniques such as nuclear magnetic resonance and high resolution mass spectrometries. Twenty-seven compounds, including phenolic acid, coumarin, lignan, terpene, alkaloid, and steroid compounds, were isolated from ethyl acetate and n-butanol fractions in water extract of Spatholobi Caulis, and they were identified as β-sitosterol(1), feruli acid methyl ester(2), syringaresinol(3),(+)-medioresinol(4),(+)-epipinoresinol(5), p-acetylphenol(6), bolusanthin Ⅳ(7), evofolin B(8), salicylic acid(9), trans-p-hydroxy-cinnamic acid(10), abscisic acid(11), m-hydroxyphenol(12), C-veratroylglycol(13), p-hydroquinone(14), 8,9-dihydroxymegastigma-4,6-dien-3-one(15), p-hydroxybenzoic acid(16), 6,9-dihydroxymegastigma-4,7-dien-3-one(17), protocatechuic acid(18), protocatechuic acid methyl ester(19), 5,7-dihydroxycoumarin(20), isolariciresinol(21), nicotinic acid(22), daucosterol(23),(+)-pinoresinol(24), stigmasterol(25), allantoin(26) and koaburaside(27), respectively. Furthermore, compounds 2-15, 19-22, 24 and 26 were isolated from genus Spatholobus for the first time.