阿萨伊油、醇提及水提物对虚热及虚寒小鼠温度趋向性及代谢水平的影响

通过考察阿萨伊油、醇提物和水提物等拆分组分对虚热及虚寒证小鼠的温度趋向性动物行为学表征,环核苷酸及代谢水平等内在生化指标的影响,探讨阿萨伊各拆分组分的寒热药性特点以及寒凉的物质基础。将昆明种雄性小鼠随机分为空白组、虚热模型组、虚热+阿萨伊冻干原粉组、虚热+阿萨伊油组、虚热+阿萨伊醇提物组、虚热+阿萨伊水提物组、虚寒模型组、虚寒+肉桂组、虚寒+阿萨伊冻干原粉组、虚寒+阿萨伊油组、虚寒+阿萨伊醇提物组、虚寒+阿萨伊水提物组共12个组。虚热组小鼠每天下午灌胃给予甲状腺片溶液160 mg·kg-1,虚寒组小鼠给予氢化可的松溶液25 mg·kg-1,连续14 d,各给药组灌胃相关药物,实验结束后测定动物温度趋向性、环核苷酸及代谢水平等相关指标。阿萨伊醇提物同阿萨伊原粉一致,表现出对虚热模型小鼠的调整作用;阿萨伊油及其水提物同肉桂一致,表现出对虚寒模型小鼠的调整作用。该研究基于中药性味可拆分理论,采用同类比较异类反证的方法证明了阿萨伊醇提物性偏凉,油及其水提物性偏温的特征,并明确了醇提物为阿萨伊性凉的物质基础。

Effect of Acaí oil,alcohol extract and water extract on temperature tendency and metabolic level of mice with deficiency-feat and deficiency-cold

To investigate the effect of resolution components, such as Acaí oil, alcohol extract and water extract, on the temperature tendency animal behavior and intrinsic biochemical indexes, such ascyclic nucleotides and metabolic level, in mice with deficiency-heat and deficiency-cold syndrome, in order to study the characteristics of the cold and heat properties of each resolution component of Acaí and the material basis of cooling. KM mice were randomly divided into 12 groups, namely blank group, deficiency-heat model group, deficiency-heat+Acaí group, deficiency-heat+Acaí oil group, deficiency-heat+Acaí alcohol extract group, deficiency-heat+Acaí water extract group, deficiency-cold model group, deficiency-cold+Cinnamomi Cortex group, deficiency-cold+Acaí group, deficiency-cold+Acaí oil group, deficiency-cold+Acaí alcohol extract group, deficiency-cold+Acaí water extract group. The mice in deficiency-heat group were given thyroid tablet solution(160 mg·kg-1), the mice in deficiency-cold group were given hydrocortisone solution(25 mg·kg-1) through gastric perfusion every afternoon for 14 days, and each administration group was given the corresponding drug. The temperature tendency, cyclic nucleotides and metabolic level of animals were measured after the experiment. The Acaí alcohol extract was consistent with the Acaí powder, with a regulatory effect on the deficiency-heat model mice;Acaí oil and its water extract were consistent with Cinnamomi Cortex, with a regulatory effect on the deficiency-cold model mice. In this study, based on the parable theory of traditional Chinese medicine′s properties and tastes, property of alcohol extract of Acaí was cool, while the property of oil and water extract were warm, the alcohol extract of Acaí was the material basis of Acaí cold medicine by the methods of homogeneous comparison and heterogeneous disproval.