| 茯苓发酵菌丝体中3种主要三萜酸类成分积累动态研究 |
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| 引用本文： | 张杨,胡高升,韩志福,萧伟,王振东,毕宇安,贾景明.茯苓发酵菌丝体中3种主要三萜酸类成分积累动态研究[J].中国中药杂志,2013,38(9):1355-1359. |
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| 作者姓名： | 张杨 胡高升 韩志福 萧伟 王振东 毕宇安 贾景明 |
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| 作者单位： | 1. 沈阳药科大学中药学院,辽宁沈阳,110016
2. 江苏康缘药业股份有限公司药物研究所,江苏连云港,222001 |
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| 摘 要： | 目的:考察茯苓发酵菌丝体中3种主要三萜酸类成分动态积累变化.方法:建立茯苓菌的液体培养方法,采用RP-HPLC测定茯苓发酵菌丝体中去氢土莫酸(DTA),3-表去氢土莫酸(eDTA)和猪苓酸C(PAC)3种主要三萜酸类成分的含量,色谱条件如下:PLATISIL ODS色谱柱(4.6 mm×250 mm,5μm)；流动相乙腈-0.5％磷酸水(80:20)；流速1.0 mL·min-1;检测波长242 nm.结果:在培养后的第8天,生物量达到最大值,但是3种主要三萜酸类成分(DTA,eDTA,PAC)的含量在培养周期内呈持续上升趋势,第17天测得3种成分质量分数分别为1.2％ (DTA),0.4％ (eDTA),1.0％ (PAC),均显著高于栽培茯苓中对应成分0.2％(DTA),0.12％ (eDTA),0.16％ (PAC)].另外,3种成分含量比例的线性回归分析结果表明,DTA与eDTA和PAC的含量比例呈显著负相关,相关系数(R2)分别为0.858 7,0.971 7.该结果表明DTA为茯苓中三萜酸类成分生合成途径中的重要中间体.结论:发酵培养17 d的茯苓菌丝体中DTA,eDTA,PAC含量之和为栽培茯苓中含量的5.55倍,说明在本发酵培养条件下,发酵培养生产茯苓中三萜酸类有效成分技术可行.
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| 关 键 词： | 茯苓 发酵 去氢土莫酸 3-表-去氢土莫酸 猪苓酸C RP-HPLC 含量测定 |
| 收稿时间： | 2012/11/3 0:00:00 |
Dynamic accumulation of three main triterpenic acids in submerged cultivation mycelium of Poria cocos |
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| ZHANG Yang,HU Gao-sheng,HAN Zhi-fu,XIAO Wei,WANG Zhen-dong,BI Yu-an and JIA Jing-ming.Dynamic accumulation of three main triterpenic acids in submerged cultivation mycelium of Poria cocos[J].China Journal of Chinese Materia Medica,2013,38(9):1355-1359. |
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| Authors: | ZHANG Yang HU Gao-sheng HAN Zhi-fu XIAO Wei WANG Zhen-dong BI Yu-an and JIA Jing-ming |
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| Institution: | School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China;School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China;School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China;Pharmaceutical Research Center, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;Pharmaceutical Research Center, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;Pharmaceutical Research Center, Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | |
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| Keywords: | Poria cocos fermentation dehydrotumulosic acid 3-epi-dehydrotumulosic acid polyporenic acid RP-HPLC content determination |
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