| 藜芦中藜芦胺的HPLC-ELSD测定方法研究 |
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| 引用本文： | 王隶书,赵大庆,陶华明,刘永宏.藜芦中藜芦胺的HPLC-ELSD测定方法研究[J].中国中药杂志,2008,33(7):791-792. |
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| 作者姓名： | 王隶书 赵大庆 陶华明 刘永宏 |
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| 作者单位： | 王隶书 (长春中医药大学,吉林,吉林,130021)； 赵大庆 (长春中医药大学,吉林,吉林,130021)； 陶华明 (吉林大学,吉林,长春,130021)； 刘永宏 (中国科学院南海海洋研究所,广东,广州,510301)； |
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| 基金项目： | 国家科技攻关计划，吉林省科技厅科技发展计划 |
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| 摘 要： | 目的:建立藜芦中藜芦胺的HPLC-ELSD测定方法。方法:采用岛津ODS-C18柱(4.6mm×250mm,4μm),以乙腈-0.1%三乙胺水溶液(50∶50)为流动相,ELSD为检测器,测定了10种不同来源藜芦中藜芦胺含量。结果:藜芦胺在0.36～3.6μg与峰面积呈良好线性关系,r=0.9998,平均回收率为100.9%(RSD2.3%,n=6)。结论:HPLC-ELSD测定藜芦胺方法简便、准确、重复性好,建立了藜芦药材含量测定项目。
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| 关 键 词： | 藜芦 藜芦胺 高效液相色谱 |
| 收稿时间： | 2007/7/16 0:00:00 |
Determination of vetatramine in Veratrum nigrum by HPLC-ELSD |
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| WANG Li-shu; ZHAO Da-qing; TAO Hua-ming; LIU Yong-hong.Determination of vetatramine in Veratrum nigrum by HPLC-ELSD[J].China Journal of Chinese Materia Medica,2008,33(7):791-792. |
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| Authors: | WANG Li-shu; ZHAO Da-qing; TAO Hua-ming; LIU Yong-hong |
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| Institution: | College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China. wls6856@163.com |
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| Abstract: | OBJECTIVE: To develop an HPLC-ELSD method for determination of vetatramine. in Veratrum nigrum. METHOD: The analy fical column was Shim-pack ODS - C18 (4.6 mm x 250 mm, 4 microm) column, the mobile phase was acetonitrile-water (containing 0.1% triethylamine) (50:50), at a flow rate of 0.8 mL x min(-1). The temperature of drift tube was 90 degrees C and the gas flow was at the rate of 2.5 L x min(-1). RESULT: The calibration curve was linear in the range of 0.36-3.6 microg (r = 0.999 8). The average recovery was 100.9% (RSD 2.3%, n = 6). The contents of veratramine in Veratrum nigrum. from the ten different sources were determined. CONCLUSION: The method may be used as a accurate and reproducible way to determine the content of veratramine in V. nigrum. |
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| Keywords: | Veratrum nigrum veratramine HPLC-ELSD |
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