丹蒲胶囊对自身免疫性前列腺炎大鼠模型炎性因子的影响

目的 观察丹蒲胶囊对自身免疫性前列腺炎大鼠模型(EAPAM)炎性细胞因子的影响，探讨中药治疗慢性前列腺炎(CP)的作用机制。方法 采用腹部皮下多点注射大鼠前列腺组织抗原的方法造成EAPAM，随机分成3组，每组10只，即：丹蒲胶囊组、前列泰片组、模型组，并以10只正常动物作为正常对照组。各组动物给予灌胃治疗，给药56天后处死动物，光镜观察各组动物前列腺组织的病理改变，通过酶联免疫吸附法检测各组动物血清及前列腺组织中 IL-8、IL- 10、TNF-α、PGE2等的水平。结果 与正常对照组比较，模型组动物血清及前列腺组织中 IL-8、、IL-10 、TNF-α水平及前列腺组织中PGE2水平均明显升高（P<0.05～0.01）。丹蒲胶囊组血清及前列腺组织中IL-8 〔（3.07±0.61）ng/L、(7．32±2.44）ng/L〕较模型组 〔（4.73±1.95）ng/L、（10.14±3.64）ng/L〕降低；丹蒲胶囊组血清及前列腺组织TNF-α 〔（85.34±19.20）ng/L 、（87.01±15.40）ng/L〕较模型组 〔（111.48±31.57）ng/L、（119.88±14.13）ng/L〕降低；丹蒲胶囊组前列腺组织中IL-10 〔（34.05±7.56）ng/L〕较模型组 〔（47.20±15.97）ng/L〕 明显降低；丹蒲胶囊组PGE2 〔（603.97±114.62）ng/L〕较模型组〔（712.58±117.10）ng/L）〕也明显降低，差异均有统计学意义（P<0.05，P<0.01）。 结论 丹蒲胶囊能够明显减轻模型大鼠前列腺炎症反应，调节局部免疫状态。

Effect of Danpu Capsule on Inflammatory Factors in Rat Model of Autoimmune Prostatitis

Objective To explore the acting mechanism of Danpu Capsule （DPC） in treating chronic prostatitis by observing its effect on inflammatory factors in autoimmune prostatitis rat model. Methods The rat model was established by abdominal subcutaneous multiple points injection of rat＇s prostate tissue antigen. Thirty modeled rats were randamly divided into 3 groups, the DPC group, the Qianlietai Tablet （QLT） group and the model group. They were treated via gastrogavage respectively with DPC, Qianlietai Tablet and normal saline respectively. Besides, a control group was set up with 10 healthy rats. All animals were sacrificed 56 days after treatment. Pathologic change of prostatic tissue was observed by light microscopy, and the levels of interleukin 8 （IL-8） , interleukin 10 （IL-10）, tumor necrosis factor α （TNF-α） and prostaglandin E2 （PGE2） in blood serum and prostatic tissue were detected by enzyme linked immunosorbent assay （ELISA）. Results Compared with the normal group, the serum and prostatic levels of IL-8, IL-10 and TNF-α, as well as the prostatic level of PGE2 in the model group were higher （P〈0.05 or P 〈0.01）. In the DPC group, the serum and prostatic levels of IL-8 was 3.07 ± 0.61 ng/L and 7.32 ±2.44 ng/L respectively, which was lower than those in the model group （4.73 ± 1.95 ng/L and 10. 14 ±3.64 ng/L, respectively） ; that of TNF-α in the DPC group （85.34 ± 19.20 ng/L and 87. O1 ± 15.4 ng/L） was also lower in the model group （ 111.48 ± 31.57 ng/L and 119.88 ± 14.13 ng/L） ; similar difference between the two groups was also shown in prostatic level of IL-IO （34.05 ± 7.56 ng/L vs 47.20 ± 15.97 ng/L） , and so was PGE2 （603.97 ± 114.62 ng/L vs 712.58 ± 117.10 ng/L） , all with statistical significance （P 〈 0.05 or P 〈 O. O1 ）. Conclusion DPC could reduce the prostatic inflammatory- response of model rats, and regulate the local immune condition.