慢性阻塞性肺疾病肺气虚证患者T淋巴细胞差异表达基因的初步研究

目的 通过基因芯片技术研究慢性阻塞性肺疾病（COPD）肺气虚证患者T淋巴细胞相关差异基因表达情况。方法 采集肺气虚证和肺阴虚证患者、健康人的外周血作为实验血样和对照血样。采用Ficoll技术分离外周血淋巴细胞、流式细胞仪分选并纯化T淋巴细胞，采用人类全基因表达谱芯片技术筛选出差异表达基因。结果 肺气虚证组／肺阴虚证组和肺气虚证组／健康对照组均高表达的差异基因15条。结论 基因芯片技术能有效地研究中医证的基因表达谱并初步筛查出COPD肺气虚证患者T淋巴细胞相关差异表达基因。

Preliminary Study on Differential Expressed Genes in T Lymphocyte of Chronic Obstructive Pulmonary Disease Patients with Fei-qi Deficiency Syndrome

ObjectiveTo study T lymphocyte related genes with differential expression in patients with chronic obstructive pulmonary disease(COPD)of Fei-qi deficiency(FQD)syndrome type by gene chips.Methods Lymphocytes in peripheral blood were isolated by Ficoll technique from blood samples collected from COPD patients of FQD syndrome type,Fei-yin deficiency(FYD)syndrome type,and also from healthy subjects for control.They were sorted and purified by flow cytometry,and the different expressed genes were screened from them by gene chip technique.Results There were 15 genes with high differential expression between patients of FQD type and those of FYD syndrome type,and between patients of FQD type and healthy subjects.Conclusion Gene chip technique could be used for studying the gene expression profiles of TCM syndrome,and the T-lymphocyte related genes with differential expression in COPD patients with FQD were screened preliminarily.