中药益髓生血颗粒对β-珠蛋白生成障碍性贫血患儿基因表达的影响

目的探讨益髓生血颗粒治疗β-珠蛋白生成障碍性贫血的作用机制。方法观察20例患儿治疗前后血红蛋白(Hb)、红细胞(RBC)、网织红细胞计数(Ret)和胎儿血红蛋白(HbF)的改变；选取3例有效病例。通过mRNA差异显示技术(DDRT—PCR)研究益髓生血颗粒对β-珠蛋白生成障碍性贫血患儿骨髓有核细胞的基因表达差异。结果与治疗前比较治疗后患儿各项血液学参数均有提高(P<0．05或P<0．01)；患儿骨髓有核细胞的铁蛋白基因表达明显降低。结论益髓生血颗粒可以改善患儿的临床症状，明显降低患儿铁蛋白基因的表达，有效缓解患儿体内铁的蓄积，这可能是益髓生血颗粒治疗本病的分子机制之一。

Effect of Yisui Shengxue Granule on Gene Expression in Children with β-Thalassemia

OBJECTIVE: To investigate the mechanism of yisui shengxue granule (YSG) in children with betathalassemia. METHODS: The changes of hemoglobin (Hb), red blood cells (RBC), reticulocyte (Ret) and fetal hemoglobin (HbF) of 20 children with beta-thalassemia were measured before and after treatment. The effect of YSG on gene expression in myelo-karyocytes in 3 selected children treated effectively by YSG was determined by using DDRT-PCR. RESULTS: The hematologic parameters were significantly enhanced and the ferritin gene expression declined after treatment respectively, as compared with those before treatment, the difference were significant (P < 0.01 or P < 0.05). CONCLUSION: YSG could improve the clinical symptoms of children with beta-thalassemia. One of its therapeutic mechanisms may be its action in decreasing ferritin gene expression so as to effectively relieve the accumulation of iron in body.