原发性高血压患者血管紧张素原基因M235T与中医证候分型的关系

目的 探讨原发性高血压患者血管紧张素原（AGT）基因M235T与中医证候分型的关系。方法 采用聚合酶链反应－限制性片段长度多态性（PCR-RFLP）方法，检测168例原发性高血压患者和42名正常人群AGT M235T基因突变频度。结果 在AGT M235T基因型突变方面，中医证型肝火亢盛型与阴阳两虚型比较，差异有统计学意义(P＜0.01)，TT型纯合子出现频率较高。通过多元线性回归分析发现原发性高血压患者的预后与AGT M235T基因变异有一定的相关性。结论 原发性高血压中医证候肝火亢盛型具有内在的分子生物学背景。

Research on Relationship between Angiotensinogen Gene M235T and TCM Syndrome Type in Essential Hypertension Patients

OBJECTIVE: To explore the relationship between angiotensinogen (AGT) gene M235T and TCM syndrome type in essential hypertension (ET) patients. METHODS: The gene mutation frequency of AGT M235T in 168 ET patients and 42 nomotensive (NT) subjects were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. RESULTS: There was a significant difference in AGT M235T gene mutation between patients of Gan-fire exuberant type and those of yin-yang deficiency type (P < 0.01), homozygote type TT appeared with higher frequency. Multivariate regression linear analysis demonstrated that the genotypes of AGT M235T was correlated with the prognosis of ET to a certain degree. CONCLUSION: Gene mutation of AGT M235T may be associated with the genesis and development of ET, and the TCM syndrome type of ET has its own intrinsic molecular biological background.