白芨愈伤组织诱导、增殖与分化研究

目的 建立白芨愈伤组织诱导、增殖和分化再生体系.方法 以MS为基本培养基,应用正交试验方法设计植物生长调节剂组合和质量浓度配比,研究不同外植体及植物生长调节剂对白芨愈伤组织形成、增殖和分化的影响.结果 白芨假鳞茎为诱导愈伤组织形成的最佳外植体；1.0 mg/L 6-苄氨基嘌呤(6-BA)与2.0 mg/L 2,4-二氯苯氧乙酸(2,4-D)组合不仅可诱导白芨愈伤组织形成,还有利于愈伤组织分化成芽.1.0 mg/L 6-BA与3.0 mg/L 2,4-D的组合促进愈伤组织增殖,增殖系数为7.8；在分化培养基中附加0.5 mg/L噻二唑苯基脲(TDZ),可提高愈伤组织分化率.结论 白芨愈伤组织诱导的最佳培养基为MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L;最佳增殖培养基为MS+6-BA 1.0 mg/L+2,4-D 3.0 mg/L;分化最佳培养基为MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L+TDZ 0.5 mg/L.

Induction, proliferation, and differentiation of Bletilla striata callus

Objective To establish the regeneration system of Bletilla striata callus for induction, proliferation, and differentiation. Methods MS was selected as the basic medium. To study the effective factors on induction, proliferation, and differentiation of callus of B. striata with different explants and to optimize the plant growth regulators, kinds, and concentration ratio of plant growth regulators by orthogonal test design. Results The optimal explants were false bulbs of B. striata. MS medium added with 1.0 mg/L 6-benzylaminopurine (6-BA) and 2.0 mg/L 2, 4-dichlorophenoxy acetic acid (2, 4-D), not only could induce callus formation, but also benefit to the differentiation of callus. MS medium added with 1.0 mg/L 6-BA and 3.0 mg/L 2, 4-D promoted the proliferation of callus, and the proliferation coefficient was 7.8. Adding with 0.5 mg/L thidiazuron (TDZ) to the differentiation medium improved the differentiation rate of callus. Conclusion MS + 6-BA 1.0 mg/L + 2, 4-D 2.0 mg/L is the optimal medium for the induction of callus; MS + 6-BA 1.0 mg/L + 2, 4-D 3.0 mg/L is the optimal medium for the proliferation of callus; MS + 6-BA 1.0 mg/L + 2, 4-D 2.0 mg/L + TDZ 0.5 mg/L is the optimal medium for the differentiation of callus.