HPLC-ESI-MS同时分析萸黄连饮片及其制剂中的活性成分

目的以萸黄连为分析对象,建立萸黄连饮片及其制剂中的多成分分析方法,阐述其"反制"炮制前后的物质基础差异,探讨萸黄连"反制"炮制的科学内涵。方法首次采用高效液相-电喷雾-三重四极杆质谱(HPLC-ESI-MS)同时测定3批萸黄连饮片,以及其制剂3批香连丸、3批香连片中绿原酸、药根碱、黄连碱、巴马汀、小檗碱、吴茱萸内酯、吴茱萸碱、吴茱萸次碱8个主要活性成分的量。结果在萸黄连饮片及其制剂中均能同时检测到黄连饮片和吴茱萸饮片中的多种成分,实验结果证明萸黄连饮片中加入了吴茱萸饮片中的多种成分。结论方法学考察结果表明此方法符合测定要求,结果准确,对阐述萸黄连的物质基础和炮制机制以及萸黄连饮片及其制剂的质量控制研究具有一定的理论意义和实用价值。

Simultaneous analysis of bioactive compounds in Yuhuanglian processing pieces and their preparations by HPLC-ESI-MS

Objective To establish the analytical method for multicomponents determination in Yuhuanglian processing pieces and preparations to explain the material basis differences before and after processing,and to approach the scientific connotation for "clash process".Methods HPLC-ESI-MS method was used for the first time to simultaneously determine chlorogenic acid,jatrorrhizine,coptisine,palmatine,berberine,evodin,evodiamine,and rutaecarpine in three batches of Yuhuanglian processing pieces,three batches of Xianglian Pill,and three batches of Xianglian Tablet.Results Several components in Coptidis Rhizoma and Evodiae Fructus processing pieces could also be found in Yuhuanglian processing pieces.So it demonstrated that several components in Coptidis Rhizoma processing pieces were added to Yuhuanglian processing pieces.Conclusion The methodology research shows that this method can fit the demand of determination and have accurate results.This is important to explain the material basis and process mechanism of Yuhuanglian and formulate the quality standard of Yuhuanglian processing pieces and preparations.