毒性中药狼毒质量标准研究

目的:建立毒性中药狼毒的质量标准。方法:采用显微、化学、液相色谱/质谱等方法进行定性鉴别,以液相色谱及液相/质谱法测定含量。结果:狼毒大戟中月腺大戟乙素含量为0.01%w/w,月腺大戟中月腺大戟乙素含量范围为0.01%~0.02%w/w,均未检出瑞香内酯、狼毒色原酮和新狼毒素A。瑞香狼毒中瑞香内酯、狼毒色原酮及新狼毒素A等成分含量范围分别为0.57%~2.12%w/w、0.86%~1.6%w/w及0.45%~0.96%w/w,但未检出含月腺大戟乙素成分。结论:各法能用于常规检测狼毒属植物。

Quality standard for Euphorbia

AIM: Euphorbia is the unprocessed root of Euphorbia ebracteolata Hayata or Euphorbia Fischeriana Steud,or Stellera chamaejasme L.To establish the quality standard for these potent herbs. METHODS: The microscope examination techniques,physio-chemical methods such as HPLC/UV and LC-MS-MS had been used. RESULTS: The content of ebracteolata compund B in E.Fischeriana was 0.01%,and in E.ebracteolata was(0.01%)-0.02%.But the skimmetine,chamaechromone and neochamaejasmin A had not been chekout in both species.The content of skimmetine,chamaechromone and neochamaejasmin A was 0.57%-2.12 %,0.86%-(1.6%),and 0.45%-0.96% in S.chamaejasme.But the ebracteolata compund B had not been chekout in this specie. CONCLUSION: The method can be applied to conventional testing of Euphorbia.