和胃安神方对失眠大鼠血浆及下丘脑食欲素A的影响

目的探讨和胃安神方对失眠大鼠血浆及下丘脑食欲素A（orexin A）的影响。方法将30只雄性wistar大鼠随机分为正常对照组、失眠模型组、和胃安神方中药组。除正常组外,其余两组大鼠均用对氯苯丙氨酸（PCPA）建立大鼠失眠模型。中药组给予和胃安神方水溶液10ml/kg灌胃,其余两组给予等量生理盐水灌胃。6天后处死并取血浆及下丘脑,采用用放射免疫分析法检测血浆及下丘脑组织orexinA的含量。采用单因素方差分析方法进行组间比较。结果和胃安神方中药组血浆orexinA含量为（22.35±4.33）pg/ml,模型组为（29.89±3.04）pg/ml,正常组为（21.28±4.02）pg/ml,中药组显著低于模型组,差异具有统计学意义（P〈0.01）,中药组与正常组比较无显著性差异（P〉0.05）;和胃安神方中药组下丘脑orexinA含量为（21.34±2.94）pg/mg,模型组为（29.75±5.05）pg/mg,正常组为（18.84±3.08）pg/mg,中药组显著低于模型组,差异具有统计学意义（P〈0.01）,中药组与正常组比较无显著性差异（P〉0.05）。结论和胃安神方治疗失眠症的疗效机制可能与其下调orexinA水平有关。

Effect of Hewei Anshen Decoction on orexin A levels in plasma and hypothalamus of insomnic rats

Objective To study the effect of Hewei Anshen Decoction（HWASD） on orexin A levels in plasma and hypothalamus in insomnic rats. Methods Thirty healthy male Wistar rats were randomly divided into three groups： normal control group, insomnia model group and HWASD treatment group. All rats but those in the normal control group were intraperitoneally injected with PCPA to be made into rat models of insomnia. The HWASD group were fed with Hewei Anshen Decoction（10 ml/kg） for 6 days, the other two groups with normal saline. After that, their plasma were collected, and hypothalamus were removed. Radioimmunoassav was used to measure the levels of orexin A in plasma and hypothalamus. Results The concentration of orexin A in the plasma of HWASD treatment group was （22.35±4.33）pg/ml, significantly lower than that of insomnia model group（P0.01）, similar with normal control group（P0.05）. The levels of orexin A in the hypothalamus of HWASD treatment group was （21.34±2.94）pg/mg, significantly lower than that of insomnia model group（P0.01）, similar with normal control group（P0.05）. Conclusion The therapeutic effect of Hewei Anshen Decoction on insomnia is partially attributed to decreased content of orexin A.