滑膜炎颗粒对2种品系小鼠镇痛作用研究

目的：比较滑膜炎颗粒在昆明小鼠和BALB/c小鼠中的镇痛作用。方法80只昆明小鼠、80只BALB/c小鼠各分为对照组、布洛芬组（0.078 g/kg）、滑膜炎颗粒大剂量组（13.98 g/kg）、滑膜炎颗粒中剂量组（6.99 g/kg）、滑膜炎颗粒小剂量组（3.50 g/kg），每组16只，雌雄各半。连续灌胃给药5 d，1次/d。第4天给药后1 h应用鼠尾光照仪，观察鼠尾受热后出现甩尾反应的潜伏期。末次给药后1 h腹腔注射醋酸制备疼痛模型，观察小鼠扭体潜伏期及扭体次数。结果滑膜炎颗粒各剂量组对昆明小鼠痛阈潜伏期、扭体次数无明显影响，滑膜炎颗粒中剂量组可减少雄性BALB/c小鼠痛阈潜伏期（3.93±0.76）min，与对照组的（4.84±1.16）min 比较，差异有统计学意义（P＜0.05）。滑膜炎颗粒小、中、大剂量组均能减少BALB/c小鼠扭体次数分别为（8.56±6.19）次、（5.73±3.17）次、（6.88±4.59）次]，与对照组的（19.06±6.34）次比较，差异有统计学意义（P＜0.01）。结论滑膜炎颗粒各剂量组对化学刺激所致疼痛呈较好镇痛作用，镇痛作用无性别差异；昆明小鼠个体差异较大，不适宜镇痛药的药效学评价，应选用个体差异小的BALB/c小鼠制备疼痛动物模型。

Comparison of analgesic effect of Huamoyan Keli between two strains of mice

Objective To compare the analgesic effect ofHuamoyan Keli（HMYKL） between Kunming mice and BALB/c mice.Methods Eighty Kunming mice and eighty BALB/c mice were randomly divided into five groups, respectively： a control group, a ibuprofen group, a HMYKL high-dose group（13.98 g crude dru g/kg）, HMYKL middle-dose group（6.99 g crude drug/kg）and a HMYKL low-dose group（3.50 g crude dru g/kg）. There were 16 mice in each group with 8 male mice and 8 female mice. Drugs were administered intragastrically daily for 5 days. After 1 h of drug treatment on day 4, the latency of tail-flick response was evaluated using illuminated pain measurement instrument. After the last drug treatment, pain model was established by i.p. acetic acid, writhing latency and writhing times were recorded to evaluate the analgesic effect of HMYKL.Results In tail-flick test, there was no statistical difference among male and female Kunming mice in the HMYKL groups. Among male BALB/c mice, the latency in HMYKL middle-dose group was significantly longer than that in the control group（4.84±1.16 minvs. 3.93±0.76 min,P〈0.05）. In writhing test, compared with control group（19.06±6.34）, the writhing times among BALB/c mice were decreased in HMYKL high-dose group（8.56±6.19）, HMYKL middle-dose group（5.73±3.17）, HMYKL low-dose group（6.88±4.59）（allP〈0.01）.Conclusion All dose groups of HMYKL showed good analgesic effect on the pain induced by chemical stimulation and there was no sex difference. Kunming mice were not suitable for the evaluation of the analgesic pharmacodynamics because of their large individual difference. On contrast, BALB/c mice which had less individual difference could be used to produce the model of pain.