电针百会、大椎对血管性痴呆模型大鼠海马区神经物质代谢及认知功能的影响

目的观察电针百会、大椎对血管性痴呆模型大鼠认知功能的影响,探讨其作用机制。方法将30只雄性SD大鼠按随机数字表法分为假手术组、模型组和电针组,每组10只。模型组和电针组大鼠采用双侧颈总动脉结扎的方法制备血管性痴呆大鼠模型。造模2周后,电针组电针百会、大椎,连续干预4周。分别于干预前和干预完成后,采用新物体识别行为学测试检测1、24 h新物体偏爱系数;氢质子磁共振波谱(1H-MRS)扫描海马区N-乙酰天冬氨酸(N-acetylaspartate,NAA)/肌酸(creatine,Cr)、胆碱(choline-containing compounds,Cho)/Cr神经代谢物水平;采用PCR法检测caspase-3、Bax mRNA表达,免疫组化检测突触素(synaptophysin,SYN)表达。结果与模型组比较,电针组1 h(69.85±11.26)%比(50.26±8.85)%]、24 h(60.85±11.86)%比(43.26±10.25)%]新物体偏爱系数均升高(P<0.01),海马区NAA/Cr(1.344±0.149)比(0.892±0.178)]水平升高(P<0.01)、Cho/Cr(0.661±0.142)比(0.902±0.178)]水平降低(P<0.01),海马区caspase-3 mRNA(1.342±0.041)比(2.437±0.089)]、Bax mRNA(2.135±0.083)比(4.358±0.137)]水平降低(P<0.01),SYN(0.577±0.035)比(0.315±0.025)]表达增加(P<0.01)。结论电针百会、大椎可有效改善血管性痴呆模型大鼠的认知功能障碍,可能与改善海马区神经代谢物NAA、Cho含量、抑制神经元凋亡及改善神经元突触功能有关。

Effect of electroacupuncture at Baihui and Dazhui on neuromaterial metabolism in hippocampus and cognitive function of rats with vascular dementia

Objective To observe the effect of electroacupuncture at Baihui and Dazhui on cognitive function of vascular dementia rats and explore its possible mechanism.Methods Male Sprague-Dawley rats were randomly divided into the sham operation group,model group and electroacupuncture group,with 10 rats in each group.Rats in the model group and the electroacupuncture group were made as vascular dementia models by carotid artery ligation of the two sides.After 2 weeks of modeling,the electroacupuncture group received electroacupuncture at Baihui and Dazhui for 4 weeks.Before and after the intervention,take the novel object recognition behavior test to detect the 1,24 h new object preference coefficient;and use magnetic resonance spectroscopy to scan the levels of NAA/Cr and Cho/Cr neurometabolites in the hippocampus.q-PCR was used to detect the expression of caspase-3 and Bax in apoptotic cells.Immunohistochemistry was used to detect synapsin SYN expression.Results Compared with the model group,the preference coefficient of new object of the electroacupuncture group at 1 h(69.85±11.26)%vs.(50.26±8.85)%]and 24 h(60.85±11.86)%vs.(43.26±10.25)%]significantly decreased(P<0.01).The content of NAA/Cr(1.344±0.149 vs.0.892±0.178)significantly increased(P<0.01),and the content of Cho/Cr(0.661±0.142 vs.0.902±0.178)in the hippocampus significantly decreased(P<0.01).The expression of hippocampus caspase-3(1.342±0.041 vs.2.437±0.089),Bax(2.135±0.083 vs.4.358±0.137)significantly decreased(P<0.01),and the expression of SYN(0.577±0.035 vs.0.315±0.025)significantly increased(P<0.01).Conclusion Electroacupuncture at Baihui and Dazhui can effectively improve the cognitive dysfunction of rats with vascular dementia,which may be related to the improvement of neuronal metabolites NAA,Cho content and inhibition of neuronal apoptosis and improvement of neuronal touch function.