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| 紫外分光光度法测定微量人参木质素的含量 | |
| 引用本文： | 李靖,程舟,杨晓伶,李珊,顾敏,万树文,张文驹,陈家宽.紫外分光光度法测定微量人参木质素的含量[J].中药材,2006,29(3):239-241. |
| 作者姓名： | 李靖 程舟 杨晓伶 李珊 顾敏 万树文 张文驹 陈家宽 |
| 作者单位： | 1. 同济大学生命科学与技术学院,上海200092 2. 上海雷允上药业有限公司,上海,200002 3. 复旦大学生命科学学院生物多样性与生态工程教育部重点实验室,上海,200433 |
| 基金项目： | 上海市科委中药现代化专项(04DZ19834) |
| 摘 要： | 目的:建立微量人参木质素含量测定的紫外分光光度法。方法:采用经典的K lason法和紫外分光光度法。结果:紫外分光光度法测得的木质素含量较K lason法高,且重复性和精密度更高。人参木质素特征性吸收峰出现在260 nm波长处。紫外分光光度法的溴乙酰处理条件为70℃保温30 m in。不同类型人参在木质素含量上有较大差异。结论:紫外分光光度法简便易行、快速准确,测定所需材料微量,适用于人参等名贵中药材的木质素含量测定。 |
| 关 键 词： | 紫外分光光度法 木质素 人参 |
| 文章编号： | 1001-4454(2006)03-0239-03 |
| 收稿时间： | 2005-10-12 |
| 修稿时间： | 2005年10月12 |
Determination of Lignin Content in Tiny Panax ginseng by UV Spectrophotometry |
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| LI Jing,CHENG Zhou,YANG Xiao-ling,LI Shan,GU Min,WAN Shu-wen,ZHANG Wen-ju,CHEN Jia-kuan.Determination of Lignin Content in Tiny Panax ginseng by UV Spectrophotometry[J].Jorunal of Chinese Medicinal Materials,2006,29(3):239-241. | |
| Authors: | LI Jing CHENG Zhou YANG Xiao-ling LI Shan GU Min WAN Shu-wen ZHANG Wen-ju CHEN Jia-kuan |
| Institution: | 1. School of Life Science and Technology, Tongji University, Shanghai 200092, China; 2. Shanghai Lei-yun-shang Medicine Co. Ltd. , Shanghai 200002, China; 3. Institute of Biodiversity Science, School of Life Science, Fudan University, Chinese Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering, Shanghai 200433, China |
| Abstract: | OBJECTIVE: To establish the UV spectrophotometry for determining lignin content in tiny Panax ginseng powder. METHODS: Classical Klason method and UV spectrophotometry were used. RESULTS: The lignin contents measured by UV spectrophotometry were higher, more repeatable and accurate as comparison with the Klason method. The specific absorptance peak of ginseng lignin appeared at 260 nm. The acetyl bromide treatment of the UV spectrophotometry was processed for ginseng powder at 70 degrees C for 30 minutes. It is also concluded that the lignin contents were obvious different among various ginsengs. CONCLUSION: UV spectrophotometry is simple, accurate and just need a little materials. It is especially suitable to determine the lignin content for ginseng and other precious Chinese traditional medicines. |
| Keywords: | UV spectrophotometry Lignin Panax ginseng |
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