三七总皂苷对脑出血大鼠凝血酶及神经功能的影响

目的：研究三七总皂苷对脑出血大鼠凝血酶及神经功能影响。方法：SD雄性大鼠200只随机分为空白组、假手术组、模型组、用药组，每组又有6 h，24 h，48 h，72 h，7天5个时间点。空白组每组5只，其余每组每个时间点10只大鼠。采用尾壳核注入胶原酶肝素生理盐水溶液法建立大鼠脑出血模型，治疗组给予三七总皂苷腹腔注射，每天一次。在相应时间点，采用改良的神经功能缺损评分（ Modified neurological severity score ，mNSS）对实验动物造模后各时间点神经功能缺损进行评价。结果：各时间点模型组mNSS和空白组假手术组比，明显升高，差异有统计学意（P＜0.05），除6 h外，各时间点模型组和用药组比，用药组mNSS明显降低，差异有统计学意义（ P＜0.05）。测定纤维蛋白原含量和纤维蛋白原凝血时间，纤维蛋白原含量和纤维蛋白原凝血时间48 h模型组和用药组差异有统计学意义（ P＜0.05）。结论：脑出血模型组中，6 h开始，mNSS明显增高，到48 h达到高峰，以后逐渐下降，三七总皂苷可以明显下调mNSS评分。纤维蛋白原含量和纤维蛋白原凝血时间在模型组升高，用药组下降，说明三七发挥了发挥了活血行淤作用。

Impact of Panax Notoginseng Saponins on the Cerebral Edema of Rats with Coagulation Cascade Pathway via Neurologic Impairment

Objective：To investigate the effects of Panax notoginseng saponins （ PNS） on the cerebral edema and thrombin in the rats with cerebral hemorrhage （ICH）.Methods：In present study, 200 SD rats were randomly divided into 4 groups： blank control, sham control , model and PNS treatment groups .ICH was induced by stereotactic injection of the collagenase Ⅳinto the right caudate nucleus . PNS or saline was injected intraperitoneally once a day after ICH , and the neurologic impairment was assessed by nMSS method for 5 time points：6 h, 24 h, 48 hour, 72 h and 7 d.Both content of serous fibrinogen （FIB） and Fibrinogen clotting time （FIBs） were measured.Results：The score of mNSS increased from 6h （P〈0.05） following ICH, and peaked at 48h, then returned to the normal on the 7th day, and the PNS decreased cerebral water content significantly at every examined time points except for 6 h （P〈0.05）. Moreover, both serous FIB and FIBs increased obviously at 48 h after ICH （P〈0.05）.The PNS inhibited the increase of both content compared with that in model group （FIB P=0.017, TT P=0.028）.Conclusion：In rats, the score of mNSS started from 6h following ICH with a peak at 48 hours, and then decreased gradually , accompanied by the elevation of FIB and FIBs at 48h.PNS can effectively alleviate the score of mNSS via the inhibition of coagulation cascade pathway .