碱性成纤维细胞生长因子和转化生长因子β受体在人巩膜成纤维细胞内的表达

目的了解人眼巩膜成纤维细胞是否表达碱性成纤维细胞生长因子(bFGF)受体FGFR1和转化生长因子(TGF-B)受体TBRⅠ和TBRⅡ。方法角膜移植后的4只眼球，应用定点解剖及游走促进法进行巩膜成纤维细胞的分离培养，建立细胞系，采用免疫荧光染色法检测bFGF受体FGFR1和。TGF-β受体TβRⅠ和TβRⅡ蛋白的表达。结果分别应用FGFR1、TβRⅠ和TβRⅡ的特异多克隆抗体染色，整个细胞表面或细胞核周呈现特异性黄绿色荧光，受体位于细胞胞膜上。肉眼观察TβRⅠ和TβRⅡ呈强阳性表达，FGFR-1表达较弱于TβRⅠ和TβRⅡ。结论人巩膜成纤维细胞表达bFGF受体FGFR和TGF-β受体TBRⅠ、TBR的功能性蛋白，巩膜是bFGF和TGF-β发挥作用的一个部位。外源的bFGF和TGF—β通过与巩膜成纤维细胞上的上述相应受体结合发挥作用，是影响实验性近视发生发展的机制之一。

Expression of bFGF receptor and TGF-beta receptors in cultured human scleral fibroblasts

OBJECTIVE: To examine the expression of basic fibroblast growth factor receptor (FGF R1) and transforming growth factor-beta receptors (TGF-beta RI and TGF-beta RII) in cultured human scleral fibroblasts. METHODS: Scleral fibroblasts of passages 2-4 were used for the present studies. Polyclonal antibodies against FGF R1, TGF-beta RI and TGF-beta RII were used to detect the proteins of these receptors. Indirect immunofluorescence staining method (IIF) was used. RESULTS: Antibodies for FGF R1, TGF-beta RI and TGF-beta RII produced specific staining of the entire cell surface, including the cell membrane enveloping cytoplasmic projections; positive staining in some cells was most intensive in the perinuclear region. Immunostaining mainly originated from the cell membranes, indicating that the presence of the receptor proteins on the cell surface. The intensity of staining for TGF-beta RI and TGF-beta RII was relatively strong, while staining of FGF R1 was relatively weak. The cells treated with PBS instead of primary antibodies did not produce specific staining. CONCLUSION: This study shows that cultured human scleral fibroblasts express the receptor protein for FGF R1, TGF-beta RI and TGF-beta RII and also indicates that these growth factors may influence these cells. Exogenous bFGF and TGF-beta administration may elicit actions through the binding with these receptors in the scleral fibroblasts.