全光谱光照对体外培养的人视网膜色素上皮细胞分泌多巴胺功能的影响

目的 探索不同光谱组成的光照射对体外培养的视网膜色素上皮（RPE）细胞分泌多巴胺功能的影响.方法 实验研究.在细胞培养箱内建立光照系统后,将体外培养的RPE细胞分别放置于无光照（对照组）、全光谱低强度光照、红绿蓝（RGB）光谱低强度光照、全光谱高强度光照、RGB光谱高强度光照环境中进行培养,连续光照24、48 h后进行检测.利用WST-1法检测各处理组之间RPE 细胞增殖率的改变,用高效液相色谱法（HPLC）检测多巴胺分泌水平.用one-way ANOVA对实验数据进行统计学分析.结果 WST-1检测结果显示：连续光照24h后,低强度光照下的全光谱光照与RGB 光谱光照对RPE细胞增殖率的影响差异无统计学意义：而在高强度光照下,全光谱光照组的RPE细胞增殖率低于RGB光谱光照组（P＜0.05）.连续光照48 h后,无论在哪种光照强度下,全光谱光照比RGB光谱光照更能抑制RPE细胞的增殖（P均＜0.01）.HPLC检测结果显示：连续光照24 h后,低强度光照下的全光谱光照与RGB光谱光照相比,对RPE细胞分泌多巴胺功能的影响差异无统计学意义；而在高强度光照下,全光谱光照组的RPE细胞分泌多巴胺的水平高于RGB光谱光照组（P＜0.01）.连续光照48 h后,同一光照强度下,全光谱光照组RPE细胞分泌多巴胺的水平高于RGB光谱光照组（P均＜0.05）.结论 光的光谱组成也是调控RPE细胞分泌多巴胺的重要因素之一,这种调控作用与光照强度和光照时间密切相关.在评估光延缓近视发生发展的作用时应该考虑光的光谱组成.

Effect of full spectral light irradiation on the dopamine secretion of cultured human retinal pigment epithelial cells in vitro

Objective To investigate the effect of different spectral compositions of light on the release of dopamine by human retinal pigment epithelial (RPE) cells in vitro.Methods Experimental study.Cultured human RPE cells (RPE-19) were exposed to either full spectrum light or red,green,blue (RGB) spectrum light in the intensities of either 4000 lx or 1000 lx.Cells exposed to no light irradiation were used as the control.Cells were assayed at 24 hours and 48 hours for the secretion of dopamine using high performance liquid chromatography (HPLC) and cell viability by WST-1 (a sodium salt of 4-3-4-iodophynyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate assay).Data were analyzed using one-way ANOVA.Results There was no statistical significance in the difference of proliferation rates between the full spectrum group and the RGB group at 1000 lx for the first 24 hours,while the difference was statistically significant at the 4000 lx level (P＜0.05).In contrast,full spectrum light significantly suppressed the proliferation of cells compared to RGB light at 48 hours,both in the low and high light intensities (both P＜0.01).After 24 hours treatment,the difference in the concentration of dopamine was not significant between the full spectrum group and the RGB group at an intensity of 1000 lx,while the difference was statistically significant at 4000 lx (P＜0.01).Furthermore,the concentration of dopamine at 48 hours was higher in the full spectrum groups than in the RGB groups at both intensity levels (both P＜0.05).Conclusion The present study found that the spectral composition of light is involved in regulating the release of dopamine by RPE and the effect was closely correlated with light intensity and time.These results demonstrate the importance of considering spectral composition when investigating sunlight's protective effect against the development of myopia.