| 睾酮对离体胎鼠头盖骨成骨细胞影响的研究 |
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| 引用本文： | 陈璐璐,曾天舒,夏文芳,柯俐.睾酮对离体胎鼠头盖骨成骨细胞影响的研究[J].中国骨质疏松杂志,1999,5(1):5-7,4. |
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| 作者姓名： | 陈璐璐 曾天舒 夏文芳 柯俐 |
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| 作者单位： | 430022,武汉,同济医科大学协和医院内分泌科 |
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| 摘 要： | 目的 探讨雄激素对成骨细胞代谢的影响。方法 胎鼠头盖骨成骨细胞培养于含不同浓度睾酮的培养基中．观察细胞胸腺嘧啶核苷掺入、碱性磷酸薛(ALP)活性、骨钙素基因表达及骨钙素合成等细胞增殖及分化等指标的变化情况。结果 胎鼠头盖骨成骨细胞的增殖不受睾酮影响，但ALP活性、骨钙素基因表达及其合成在不同程度上受睾酮的调节。结论 雄激素对成骨细胞的分化具有促进作用，但对其增殖无影响。
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| 关 键 词： | 成骨细胞 睾酮 胎鼠 骨钙素 雄激素 离体 影响 增殖 基因表达 ALP |
Effects of testosterone on fetal rat calvarial osteoblastic cells in vitro |
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| Chen Lulu,Zeng Tianshu,Xia
Wenfang,et al.Effects of testosterone on fetal rat calvarial osteoblastic cells in vitro[J].Chinese Journal of Osteoporosis,1999,5(1):5-7,4. |
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| Authors: | Chen Lulu Zeng Tianshu Xia
Wenfang |
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| Institution: | Chen Lulu,Zeng Tianshu,Xia
Wenfang,et al
Department of Endocrinology,Union Hospital,Tongji Medical University,Wuhan 430022,China |
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| Abstract: | Objective\ To investigate the effect of testosterone on bone cell metabolism.Methods\ Fetal rat calvarial osteoblastic cells were cultured in the medium containing different concentrations of testosterone. 3H thymidine incorporation,alkaline phosphatase(ALP) activity,osteocalcin gene expression and production were detected to determine the condition of cell proliferation and differentiation. Results \ The proliferation of fetal rat calvarial osteoblastic cells was independent of the presence of testosterone.However,ALP activity,osteocalcin gene expression and production were regulated in different extent by testosterone. Conclusion\ Testosterone stimulates the differentiation of rat calvarial osteoblastic cells in vitro . |
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| Keywords: | Testosterone \ Fetus\ rats Osteoblastic cells\ Proliferation \ Differentiation |
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