获得性免疫缺陷综合征儿童抗病毒治疗后外周血单个核细胞线粒体DNA基因D环区序列研究

目的研究我国人类免疫缺陷病毒（HIV）感染儿童长期抗反转录病毒治疗（ART）后对外周血单个核细胞（PBMC）线粒体DNA基因D环（D-loop）区序列的影响。方法选择34例ART的HIV感染儿童与21例正常对照儿童,提取两组儿童PBMC内DNA,普通聚合酶链反应（PCR）法扩增线粒体DNA D-loop区序列并测序。结果 ART组儿童PBMC内线粒体DNA D-loop区碱基变异数（6.29±2.25）bp高于对照组儿童（4.71±2.53）bp（P〈0.05）,ART组患儿PBMC线粒体DNA D-loop区共有50个位点出现碱基改变,其中有3个位点的变异频率在两组儿童间差异有统计学意义,即16362T→C、131T→C、489T→C。结论 ART可能导致儿童PBMC线粒体DNA Dloop区基因变异增加。

Research on mitochondrial DNA D-loop sequences of peripheral blood mononuclear cells in HIV-1 infected children after antiretroviral therapy

Objective The study was to estimate the mitochondrial DNA D-loop region sequences of peripheral blood mononuclear cells（PBMC）in human immunodeficiency virus 1（HIV-1）infected children receiving long-term antiretroviral therapy（ART）.Methods Thirty-four ART-treated HIV-infected children and 21healthy children were included in the study,and D-loop regions were amplified by polymerase chain reaction after PBMC DNA was extracted from all of the children.Results Base variable sites numbers of PBMC mitochondrial DNA D-loop in ART-treated children（6.29±2.25）bp were higher than those of control children（4.71±2.53）bp.In ART-treated children,there were 50nucleotide variation sites found in D-loop region,and only three sites variation frequency difference between the two groups were statistically significant（P 0.01）,i.e.16362T→C,131T→C,489T→C.Conclusion ART may cause more gene variations in mitochondrial DNA D-loop regions.