蝎毒多肽提取物对小鼠S_(180)肉瘤和H_(22)肝癌血管生成抑制作用的实验研究

目的 :在器官和体内水平观察东亚钳蝎蝎毒的多肽提取物 (PESV)的抗血管生成活性和对肿瘤生长的抑制作用。方法 :①观察PESV对鸡胚尿囊膜 (CAM )新生血管生成的影响。②观察皮下注射PESV(0 .3mg/kg)对S180 肉瘤和H2 2 肝癌荷瘤小鼠肿瘤生长和肿瘤血管生成的影响。③用免疫组化的方法观察PESV对S180 肉瘤和H2 2肝癌荷瘤小鼠肿瘤组织血管内皮细胞生长因子VEGF和bFGF表达的影响。结果 :0 .5mg/CAM和 0 .8mg/CAM的PESV能明显抑制CAM新生血管的形成。PESV能明显抑制小鼠S180 肉瘤和H2 2 肝癌的肿瘤生长和血管生成水平 ,并降低肿瘤组织内血管生成相关因子VEGF和bFGF的表达。结论 :PESV具有良好的体内和体外抗肿瘤血管生成活性 ,并藉此抑制肿瘤的生长。

EXPERIMENTAL STUDY OF POLYPEPTIDE EXTRACT FROM SCORPION VENOM ON S180 SARCOMA AND H22 HEPATOMA IN MICE

Objective:the study was designed to evaluate the anti-angiogenic activity and anti-tumor activity in vivo of PESV,a peptide extract from scorpion venom.Methods:PESV was isolated by Sephadex G50 gel filtration with Sepharose FF ion-exchange chromatography. Chicken embryo chorioallantoic membrane (CAM) assay was used to determine the effect of PESV on neovascularization in vivo.The mice with S 180 sarcoma or H 22 hepatoma were injected with PESV at 3 mg/kg,angiogenesis in vivo and tumor growth were observed.Results: PESV demonstrated suppression of neovascularization in the CAM assay.In vivo,systemic administration of PESV suppressed tumor growth of implanted S 180 sarcoma or H 22 hepatoma in mice. Immunohistochemical examination showed a significant decrease in the number of blood vessel and reduced expression of VEGF and bFGF by PESV.Conclusions:PESV is of potent anti-angiogenic activity in vivo. PESV could be regarded as a candidate for tumor angiogenesis inhibitor (TAI) and a potent chemotheraprutic agent of malignant tumors, and its component should be further analyzed.