氧化吲哚类化合物Z24抑制ECV304细胞增殖及迁移的实验研究

目的探讨氧化吲哚类化合物Z24对血管内皮细胞增殖及迁移的抑制作用.方法利用3H-TdR掺入DNA方法来检测Z24对人脐静脉内皮细胞ECV304增殖的影响;扫描电镜方法测定Z24对细胞骨架系统的影响;体外细胞迁移实验测定它对内皮细胞迁移的抑制效应;用免疫沉淀几Western印迹法检测它对ERK信号通路的影响.结果Z24能抑制EGF刺激后ECV304细胞的增殖,能显著抑制ECV304的骨架形成;ECV304的迁移减少;p-ERK水平下调.结论Z24能够抑制血管内皮细胞的增殖和迁移.这种抑制效应可能阻断MAPK信号通路发挥作用的.

The inhibitory effects of an indole 2-Ketone compound Z24 on ECV304 cell proliferation and migration

Aim To study the inhibitory effects of an indole 2-ketone compound Z24 on proliferation and migration of vascular endothelial cell. Methods The incorporation of 3H] thymidine was used to detect the effect of Z24 on the proliferation of ECV304 cell. The whole cell scanning electron microscopy was used to determine the effect of Z24 on cytoskeleton of ECV304 cell. The cell migration experiment was also used to evaluate the effect of Z24 on ECV304 cell, and Western blotting detected the phosphorylation of ERK in ECV304 cell treated by Z24. Results The DNA synthesizing of ECV304 cell was inhibited and the cytoskeletone of ECV304 cell was wrinkled by Z24. The cell migration was found evidently to be reduced in Z24 treated ECV304 cell. A lower level expression of phosphorylation of ERK was found ECV304 cell exposed to Z24. Conclusions Z24 might inhibit ECV304 cell proliferation and migration. It was suggested the inhibited effect of z24 might be to work through MAPK signal pathway.