铅诱发蚕豆根尖细胞的遗传损伤

目的研究醋酸铅对蚕豆根尖细胞的遗传损伤效应。方法对籽粒饱满的蚕豆种子催芽24h后选根长1.0～1.5cm的蚕豆随机分组,用浓度为0.1、1.0、5.0、25.0、50.0mg/L的醋酸铅溶液处理,时间分别为12、24、36h,进行蚕豆根尖细胞微核试验。结果醋酸铅溶液可诱发蚕豆根尖细胞微核率显著升高(P<0.01),浓度超过0.1mg/L还可明显降低蚕豆根尖细胞有丝分裂指数(P<0.05),且高浓度短时间作用和低浓度长时间作用具有等效性。结论醋酸铅呈浓度依赖和时间依赖地显著降低蚕豆根尖细胞的有丝分裂指数,并诱发蚕豆根尖细胞微核率显著升高,利用蚕豆根尖细胞微核技术检测环境铅污染是可能的。

The Effects of Lead on Root Tip Cell of Vicia faba

Objective To study the genetic effects of lead on root tip cell of Vicia faba. Methods Micronucleus tests were conducted on root tip cell of Vicia faba treated with Pb2+ of concentrations of 0.1, 1.0, 5.0, 25.0, 50.0 mg/L for 12-36 h. Results The results indicated that the rates of micronucleus of lead-treated tip cell were significantly higher than that of the control (P<0.01),the mitotic index significantly decreased compared with the control (P<0.05)and the response of high dose-short time exposure was the same as that of low dose-long time exposure. Conclusion Lead may induce, concentration-dependent and time-dependent, rate of micronucleus increasing in Vicia faba root tip cell and mitotic index decreasing as well. It is feasible to monitor lead pollution using micronucleus test on root tip cell of Vicia faba.