姜黄素对子宫颈癌HeLa细胞增殖抑制作用及其机制的研究

目的:探讨姜黄素(Curcum in)对体外培养人子宫颈癌HeLa细胞抗癌作用及分子机制。方法:MTT法检测细胞增殖,流式细胞仪检测凋亡和细胞周期,PI/Hoechst33258荧光双染法检测细胞凋亡,W estern b lot法检测细胞凋亡相关蛋白Bc l-2和Bax蛋白的表达。结果:姜黄素对HeLa细胞生长有抑制作用,并呈剂量依赖性;流式细胞仪分析证实姜黄素能使HeLa细胞阻滞在S期,并出现亚二倍体凋亡峰;荧光双染法可见凋亡细胞;W estern b lot结果显示Bax蛋白表达均上调,而Bc l-2的表达无明显影响。结论:姜黄素对人子宫颈癌HeLa细胞的增殖具有显著的抑制作用并可诱导细胞凋亡;Bax蛋白的表达上调可能参与了诱导细胞凋亡。

Effect of anti-proliferation of curcumin on cervical carcinoma HeLa cells and its mechanism

Objective:To investigate the mechanism of anti-tumor effects of curcumin on cervical carcinoma HeLa cells.Methods: Growth inhibition rates of HeLa cells were measured with MTT method.The cell cycle distribution and apoptosis were detected by flow cytometic analysis.In addition,apoptosis was detected by Hoechst3358/PI fluorescent staining and the expression of Bcl-2 and Bax protein were detected by using western blot assay.Results: Curcumin inhibited the proliferation of HeLa cells in a does-and time-dependent manner.The cell growth was arrested at S stage.And aneuploid peak was observed by FCM.Fluorescent staining can detect the apoptotic cells.The expression of Bax was increased and the Bcl-2 was unchanged.Conclusion: Curcumin not only inhibits proferation but also induces apoptosis of HeLa cells.The possible mechanism is related with the increasing of Bax.