Vanin对胰岛NIT细胞保护机制的探讨

目的探讨泛酰巯基乙胺酶Vanin对胰岛NIT细胞的保护作用及机制。方法培养胰岛B细胞株NIT细胞，以5ng／ml IFN-1，50pg／ml IL-1β，10ng／ml Vanin处理细胞，分为IFN-γ＋IL-1β3＋Vanin组、IFN-γ＋IL-1β组、Vanin组、对照组（DMEM）。4组干预因素分别作用于对数生长期细胞24h后采用MTF法检测各组NIT细胞的增殖抑制率，化学发光法检测上清液中胰岛素（Ins）水平，硝酸还原酶法检测上清液中一氧化氮（N0）水平。结果经IFN-γ＋IL-1β处理的NIT-1细胞增殖受抑制，抑制率为60．11％；予Vanin预处理组细胞增殖抑制率为36．98％，较IFN-γ＋IL-1β处理组降低（P〈O．01）。经IFN-γ＋IL-1β破坏的NIT-1细胞分泌胰岛素较其他3组减少（P〈O．05，P〈0．01），NO产生较其他3组增多（P〈0．05，P〈0．01）。而接受Vanin预处理组再予IL-1β＋IFN-γ破坏的NIT-1细胞与IL-1β＋IFN-γ叫组比较，分泌胰岛素增加（P〈0．05）、NO水平降低（P〈0．05）。结论Vanin可减轻IFN-γ＋IL-1β对NIT细胞的损伤作用，保护NIT细胞的胰岛素分泌功能。

Protective Effect and Mechanism of Vanin on Islet NIT Cells

Objective To investigate the protective effect and mechanism of pantetheinase Vanin on islet NIT cells. Methods NIT cells of Islet βcell line in four groups were respectively cultivated with one of the following components： IFN-γ＋IL-1β＋Vanin, IFN-γ＋IL-1β ,Vanin, DMEM. The concentration were fixed by preliminary experiment： IFN-γ5 ng/ml, IL-1β3 50 pg/ml, Vanin 10 ng/ml. Inhibition ratio of proliferation were tested with MTY assay by different interventions on NIT cells. Insulin levels in supernatants were tested with chemiluminescence assay and nitric oxide levels in supernatants were tested with nitrate reductase assay. Results NIT cells proliferation rate were inhibited by 60.11% with IFN-γ＋IL-1β while with Vanin pretreatment, the inhibition ratio declined to 36.98% （P〈0.01）. Insulin concentration in supeinatants ofIFN-γ＋IL-1β group were lower than the other three groups （P〈0.05, P〈0.01）, while NO concentration higher than the other three groups （P〈0.05, P〈0.01）. Insulin concentration increased （P〈0.05） and NO decreased （P〈0.05） with Vanin pretreatment than those of IL-1β＋IFN-γgroup. Conclusion Vanin alleviates the damage of IFN-γ combined with IL-1β 3 on NIT cells and protects insulin secretion function of NIT cells.