姜黄素抗兔动脉粥样硬化作用的研究

目的：研究姜黄素抗兔动脉粥样硬化（AS）的作用机制。方法：30只雄性日本大耳白家兔，其中给予高脂饲料喂食2个月，复制AS模型家兔20只，随机分成治疗和对照两组，各10只。均予基础饲料喂养，治疗组每天给予姜黄素（200mg／kg）灌胃，对照组每天给予生理盐水灌胃，共治疗1个月。治疗后处死家兔，观察两组主动脉粥样斑块面积比；取主动脉弓做病理切片；免疫组织化学检测主动脉壁主动脉基质金属蛋白酶9（MMP-9）和核转录因子-κB（NF-κB）的表达。结果：治疗组斑块面积／主动脉面积比低于对照组（P〈0．05）。免疫组化显示，治疗组MMP-9、NF-κB阳性细胞表达率明显低于对照组，分别为（16．5±8．0）％VS（38．5±13．6）％，P〈0．05；（22．61±7．95）％VS（42．28±19．34）％，P〈0．05]。结论：姜黄素可以显著降低动脉粥样硬化家兔主动脉壁MMP-9、NF-κB的表达，抑制动脉粥样硬化的形成。

The Effect of Curcumin on Resisting Atherosclerosis in Rabbits

Objective：To investigate the effect of eureumin on resisting atheroselerosis in the aorta atheroselerotie Rabbits. Methods：Thirty male Japanese White rabbits were fed with a high cholesterol diet for two consecutive months. Finally, 20 rabbits were established atherosclerotic models. They are randomly divided into two groups： the control group （10 animals） and the treatment group （ 10 animals）, Both groups were fed with a standard diet in the following month. Additionally, the treatment group received an oral curcumin at a dose of 200mg/Kg body wt and the control group received an oral curcuminfree sodium chloride solution daily during experiment. At the end of therapy period, all animals were killed. Entire aorta was rapidly dissected out and the area ratio of atherosclerotic plaque/aorta was examined. The aortic arch was selected for histological examinations and stained with hematoxylin and eosin （H＆E）. The expressions of NF-κB and MMP-9 in aorta were examined with semiquantitative analysis immunohistochemistry. Results：Finally, each of the two groups had eight rabbits selected into analysis. The area ratio of plaque/aorta in the treatment group reduced obviously compared with those of control group（ P 〈 0.05 ）. The results of immunohistochemistry manifested the expression ratio of NF-κB and MMP-9 positive cells in treatment group were significantly lower than those in control group （ 16.50 ± 8.00 ） % vs （ 38.50±3.60 ） %, P 〈 0.05 ; （ 22.61±7.95 ） % vs （ 42.28±9.34 ） %, P 〈 0.05 ; Respectively ]. Conclusion： Curcumin can decrease the expression of nuclear factor kappa-B and matrix metalloproteinase-9 in aorta and attenuate atherosclerotic development in rabbits.