癌性胸腔积液端粒酶活性的检测

目的：探讨端粒酶活性水平检测对癌性胸腔积液的诊断价值。方法：采用Telomerase-PCR-ELISA法检测了30例癌性胸腔积液及21例良性胸腔积液的端粒酶活性水平，并与细胞学检查比较结果：癌性胸腔积液端粒酶活性阳性率76．6％，结核性胸腔积液20％（3／15），其他病因胸腔积液0％（0／6），癌性胸腔积液端粒酶活性高于细胞学检查率36．6％（P＜0．005），两者平行试验阳性率83．3％。结论：端粒酶活性的检测可能成为诊断癌性胸腔积液的重要辅助手段。

Detection of Telomerase Activity in Pleural Effusion Cells from Patients with Malignant Neoplasm

Objective:To investigate the diagnostic significance of the detection of telomerase activity in pleural effusion cells from patients with malignant neoplasm. Methods:The technique telomerase polymerase chain reaction(PCR)-enzyme-linked immunosorbent assay (ELISA) was employed to detect telomerase activity in 30 samples of pleural effusion cells of patients with malignant neoplasm, 20 samples of tuberculotic patients and 6 samples of patients with other diseases. Results:It was found that the positive rate of telomerase activity in samples from patients with malignant diseases were higher (76.6%) than that from tuberculotic patients(20%) or other patients(0%). The positive rate of telomerase activity in samples from patients with malignant diseases was much higher than that of cytologic measure. The combining positive rate of the two examinations was 83.3%. Conclusions:Detection of telomerase activity in pleural effusion cells would be helpful to the cytologic measure in diagnosis of patients with malignant neoplasm.