复方参芎滴丸中川芎提取纯化工艺

目的:考察复方参芎滴丸中川芎的提取、纯化工艺条件。方法:以阿魏酸、藁本内酯提取量为指标,通过正交试验考察乙醇体积分数、溶剂用量及提取时间对川芎提取工艺的影响,利用单因素试验考察提取液质量浓度、溶剂用量及提取次数对川芎提取液纯化工艺的影响。采用HPLC测定阿魏酸、藁本内酯含量,流动相甲醇-0.05%磷酸水溶液梯度洗脱,检测波长320 nm。结果:川芎最佳提取工艺为加10倍量70%乙醇提取2次,每次2 h;阿魏酸、藁本内酯提取量分别为1.46,13.84mg·g-1。最佳纯化工艺为加2倍量乙酸乙酯萃取3次;阿魏酸、藁本内酯转移率分别为94.68%,85.86%。结论:优选的提取、纯化工艺条件稳定可行,活性成分提取率高,可为复方参芎滴丸的工业化生产提供参考。

Extraction and Purification Process of Chuanxiong Rhizoma in Compound Shenxiong Dropping Pills

Objective: To optimize extraction and purification procedure of Chuanxiong Rhizoma in compound Shenxiong dropping pills. Method: Ethonal extraction of Chuanxiong Rhizoma was conducted through orthogonal design with contents of ferulic acid and ligustilide as indexes,solvent extraction method was used to purify Chuanxiong Rhizoma extract on the basis of single factor experiments,effects of different concentration,solvent volume and extraction times were studied.Contents of ferulic acid and ligustilide were determine by HPLC,methanol-0.05% phosphoric acid as mobile phase in a gradient elution,detection wavelength was 320 nm. Result: Optimum extraction procedure was that medicinal materials were extracted 2 times with 10 times the amount of 70% ethanol,2 hours for each time.Contents of ferulic acid and ligustilide in extract were 1.46,13.84 mg · g^-1.Diversion rate of ferulic acid and ligustilide were 94.68% and 85.86%.Purification procedure was carried out by extracted 3 times with 2 times the amount of ethyl acetate for each time. Conclusion: Extraction rate of active components is high by optimized extraction and purification procedure,which can provide experimental basis for industrial production of compound Shenxiong dropping pills.