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| 马尾松花粉多糖硫酸酯化前后生物活性的比较研究 | |
| 引用本文： | 耿越,刘辉,石丽花,刘志河.马尾松花粉多糖硫酸酯化前后生物活性的比较研究[J].中国生化药物杂志,2010,31(2). |
| 作者姓名： | 耿越 刘辉 石丽花 刘志河 |
| 作者单位： | 1. 山东师范大学,生命科学学院,动物抗性生物学重点实验室,山东,济南,250014 2. 山东省济南市疾病控制中心,山东,济南,250001 3. 烟台新时代天然营养品有限责任公司,山东,烟台,264000 |
| 摘 要： | 目的对马尾松花粉多糖(PPM)进行硫酸酯化,得硫酸酯化多糖(S-PPM),比较酯化前后抗肿瘤活性、免疫调节活性和清除自由基能力的变化。方法PPM采用氯磺酸-吡啶法进行硫酸酯化。结果S-PPM取代度为1.47,S-PPM对体内外肿瘤细胞的抑制作用、对促进T、B细胞增殖作用均高于PPM;对促进巨噬细胞的吞噬作用变化不大;S-PPM清除超氧阴离子自由基的作用明显强于PPM,清除羟自由基的作用小于PPM。结论S-PPM能促进特异性免疫抑制肿瘤细胞的增殖;硫酸酯化也能改善对多糖清除自由基的作用。 |
| 关 键 词： | 多糖 马尾松花粉 硫酸酯化 抗肿瘤 免疫调节 自由基 |
Comparative study on bio-activity of polysaccharide from Pinus massoniana pollen and its sulfated derivative |
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| GENG Yue,LIU Hui,SHI Li-hua,LIU Zhi-he.Comparative study on bio-activity of polysaccharide from Pinus massoniana pollen and its sulfated derivative[J].Chinese Journal of Biochemical Pharmaceutics,2010,31(2). | |
| Authors: | GENG Yue LIU Hui SHI Li-hua LIU Zhi-he |
| Institution: | GENG Yue1,LIU Hui2,SHI Li-hua3,LIU Zhi-he3(1.School of Life Science,Provincial Key Lab of Animal Resistant Biology,Sh,ong Normal University,Jinan 250014,China,2.Jinan Municipal Center for Disease Control & Prevention,Jinan 250001,3.New Era Health Industry International Development Co.,Ltd.,Yantai 264000,China) |
| Abstract: | Purpose To study the polysaccharide from Pinus massoniana pollen (PPM) and to compare its anti-tumor,immune modulation activities and scavenging qualities of free radical with its sulfated derivative (S-PPM).Methods PPM was chemically modified by chlorosulfonic acid-pyridine method and their bioactivities were compared.Results The substituting degree of S-PPM was 1.47.Results showed that S-PPM was more powerful in inhibiting the growth of tumor cell in vivo and in vitro and in promoting T,B lymphocytes than... |
| Keywords: | polysaccharide Pinus massoniana pollen sulphate anti-tumor immunity modulation free radical |
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