| 正交试验优化大鼠糖尿病胃轻瘫模型 |
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| 引用本文： | 吴国泰,杜丽东,景琪,高云娟,刘五州,李应东,任远.正交试验优化大鼠糖尿病胃轻瘫模型[J].中草药,2014,45(14):2047-2050. |
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| 作者姓名： | 吴国泰 杜丽东 景琪 高云娟 刘五州 李应东 任远 |
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| 作者单位： | 甘肃中医学院 药理学教研室, 甘肃 兰州 730000;甘肃省中药药理与毒理学重点实验室, 甘肃 兰州 730000;甘肃中医学院 药理学教研室, 甘肃 兰州 730000;甘肃省中药药理与毒理学重点实验室, 甘肃 兰州 730000;甘肃中医学院 药理学教研室, 甘肃 兰州 730000;甘肃中医学院 药理学教研室, 甘肃 兰州 730000;甘肃中医学院 药理学教研室, 甘肃 兰州 730000;甘肃省中药药理与毒理学重点实验室, 甘肃 兰州 730000;甘肃中医学院 药理学教研室, 甘肃 兰州 730000;甘肃省中药药理与毒理学重点实验室, 甘肃 兰州 730000 |
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| 基金项目： | “十二五”国家科技支撑计划项目子课题（2011BAI05B0206）；甘肃省自然科学基金项目（1212RJZA079）；甘肃省中药药理与毒理学重点实验室开放基金项目（ZDSYS-KJ-2012-004） |
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| 摘 要： | 目的 通过正交试验优化糖尿病胃轻瘫大鼠模型的制备方法。方法 采用四氧嘧啶（ALX）法复制大鼠糖尿病胃轻瘫模型；采用4因素3水平的正交试验设计，以空腹血糖、胃内残留率和血浆胃动素（MOT）水平综合评分为评价指标，观察不同影响因素对大鼠糖尿病胃轻瘫模型形成的影响。结果 饲料类型对大鼠糖尿病胃轻瘫模型的影响不明显，造模时间、ALX剂量对大鼠糖尿病胃轻瘫的形成有决定性影响，3个影响因素重要性依次为造模时间>ALX剂量>饲料类型。结论 通过优化试验方法，可有效提高糖尿病胃轻瘫大鼠模型的造模效果，为科学评价糖尿病胃轻瘫的药物研究提供技术平台。
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| 关 键 词： | 糖尿病胃轻瘫模型 糖尿病大鼠 残留率 造模 影响因素 大鼠模型 饲料类型 四氧嘧啶 试验优化 正交试验设计 |
| 收稿时间： | 9/7/2013 12:00:00 AM |
Optimization of diabetic gastroparesis rat model by orthogonal design |
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| WU Guo-tai,DU Li-dong,JING Qi,GAO Yun-juan,LIU Wu-zhou,LI Ying-dong and REN Yuan.Optimization of diabetic gastroparesis rat model by orthogonal design[J].Chinese Traditional and Herbal Drugs,2014,45(14):2047-2050. |
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| Authors: | WU Guo-tai DU Li-dong JING Qi GAO Yun-juan LIU Wu-zhou LI Ying-dong and REN Yuan |
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| Institution: | Department of Pharmacology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China;Key Laboratory of Pharmacology and Toxicology of TCM of Gansu Province, Lanzhou 730000, China;Department of Pharmacology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China;Key Laboratory of Pharmacology and Toxicology of TCM of Gansu Province, Lanzhou 730000, China;Department of Pharmacology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China;Department of Pharmacology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China;Department of Pharmacology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China;Key Laboratory of Pharmacology and Toxicology of TCM of Gansu Province, Lanzhou 730000, China;Department of Pharmacology, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China;Key Laboratory of Pharmacology and Toxicology of TCM of Gansu Province, Lanzhou 730000, China |
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| Abstract: | Objective To optimize the preparation methods of diabetic gastroparesis rat model by orthogonal test. Methods The diabetes gastroparesis rat model was duplicated with alloxan (ALX) by using four factors and three levels of orthogonal design. The fasting blood glucose, gastric emptying rate, and plasma motilin level were scored as evaluation indexes, and the influence of different factors on diabetic gastroparesis model rats was observed. Results The effect of feed type on the diabetic gastroparesis rat model was not obvious, but the effects of modeling time and modeling agent ALX dose on diabetic gastroparesis rat model were decisive. The order of the importance was as follows: modeling time > dose of ALX > feed types. Conclusion The optimized test method can effectively improve the diabetic gastroparesis rat model and provide a technical platform for the study on drug evaluation of diabetic gastroparesis. |
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| Keywords: | alloxan diabetic gastroparesis orthogonal test motilin model |
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