异常剪切应力促兔颈总动脉粥样硬化模型的建立

目的建立异常剪切应力促兔颈总动脉粥样硬化（As）动物模型，论证不同程度As病变与不同程度和形式的剪切应力相关，为研究异常剪切应力在As中的作用奠定基础。方法给予26只新西兰兔右颈总动脉硅胶管套环后等分为对照组（给予普通饮食）和高脂组（2％高胆固醇饮食）；9周后处死动物，第。周和第9周收集血清测量总胆固醇（Tc）、低密度脂蛋白胆固醇（LDLC）、高密度脂蛋白胆固醇（HDLC）、甘油三酯（TG）水平；采集两侧颈总动脉，苏丹Ⅳ和HE染色观察颈总动脉As斑块面积。结果高脂组血清TC、LDLC和HDLC水平明显较对照组高（P〈0．05）。颈总动脉苏丹Ⅳ染色和HE染色显示：高脂组远心段和近心段都有斑块形成，且近心段斑块更加明显，并有大量泡沫细胞堆积。结论成功构建了异常剪切应力促兔颈总动脉As模型，并提示不同节段不同程度As病变与异常剪切应力相关。

Establishment of New Zealand Rabbits Carotid Atherosclerosis Model Induced by Abnormal Shear Stress

Objective To build the rabbits model with carotid atherosclerosis produced by abnormal shear stress and demonstrate that different levels and forms of shear stress in the casts, upstream from the casts, and downstream from the casts in the carotid arteries can lead to different levels of carotid atherosclerosis. Methods Assembled with silastic collars around the right carotid arteries,all the New Zealand white rabbits were randomly divided into two groups, the control group （n = 13 ） and the high-cholesterol group （n = 13）. The rabbits in the high-cholesterol group were fed with a high-cholesterol diet （2% cholesterol wt/wt） for 9 weeks. Results Plasma total cholesterol （TC）, low density lipoprotein cholesterol （ LDLC）, and high density lipoprotein cholesterol（HDLC） concentrations were markedly increased in the high-cholesterol group by enzymatic methods. More plaques were identified in the upstream than the downstream with Sudan IV and HE staining. Conclusion The rabbits model with carotid atherosclerosis produced by abnormal shear stress was built. These combined findings suggest that different levels and forms of shear stress can lead to different carotid atherosclerosis lesions.