膀胱移行细胞癌PCNA的表达及其意义

目的 :探讨膀胱移行细胞癌增殖细胞核抗原 (PCNA)的表达与肿瘤病理分级、临床分期、预后的关系。方法 :采用免疫组化S P法检测 6 5例膀胱移行细胞癌和 11例正常膀胱组织PCNA的表达。结果 :PCNA在膀胱癌及正常膀胱组织的表达差异有显著性 (P <0 .0 1) ;随着膀胱癌病理分级、临床分期升高 ,PCNA阳性表达上升 ,Ⅰ级、Ⅱ级与Ⅲ级比较 ,PCNA的阳性表达差异均有显著性 (P <0 .0 1) ;T2 ～T4 期PCNA的阳性表达显著高于Tis～T1期 (P <0 .0 0 5 ) ;术后 5年内无复发组PCNA的阳性表达显著低于术后 5年内复发组 (P <0 .0 0 5 )。结论 :PCNA可作为膀胱癌恶性程度及预后判断的指标之一 ,可指导临床综合治疗

Expression of PCNA in human bladder transitional cell carcinoma and its clinical significance

Objective:To investigate the expression of P CNA in human bladder transitional cell carcinoma(TCC) and its relationship with the hi stological grades,clinical stages and prognosis of TCC.Methods:PCNA expression was detected by S-P immunohistochemistry me thod in 65 TCC cases and 11 normal bladder tissues.Results:The expression of PCNA between TCC group and normal tissue g roup was significantly different(P＜0.01).The poorer the differentiation of bladder cancer was related to the higher level of the PCNA positive expression. The c arcinoma of (Ⅰ,Ⅱ)grades,(Tis～T\-1) stages exhibited lower PCNA expression lev el as compared with Ⅲ grade,(T\-2～T\-4) stages respectively(P＜0.005).The expression of PCNA in recurrence group was significantly higher than that in no nrecurrence group(P＜0.005).Conclusions:PCNA could be used as a marker in the determination of t he malignancy,therapy and prognosis of TCC.