| 输血传播病毒样微小病毒基因检测 |
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| 引用本文： | 刘元元,马振华,王峰,牛俊奇,于永利.输血传播病毒样微小病毒基因检测[J].吉林大学学报(医学版),2006,32(6):1045-1047. |
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| 作者姓名： | 刘元元 马振华 王峰 牛俊奇 于永利 |
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| 作者单位： | 1.吉林大学第一医院感染症科,吉林 长春 130021；2.吉林大学基础医学院免疫学教研室，吉林 长春 130021 |
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| 基金项目： | 国家自然科学基金
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卫生部博士点专项基金
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吉林省科技厅科研项目 |
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| 摘 要： | 目的：测定慢性肝炎患者体内输血传播病毒(TTV)样微小病毒（TLMV）的全基因序列，并研究人群中TLMV感染状况。方法：采用日本学者Shunji Mishiro提供的序列选择TLMV-CBD279和CBD-231最保守区设计引物，用慢性乙型肝炎且TTV阳性患者血清制备DNA模板，进行PCR扩增；将阳性PCR产物连接至pGEMR-T质粒，碱裂解法提取质粒DNA并纯化。以T7 引物为起
始全自动荧光测序仪测序。结果：获得162bp基因序列，该序列与日本株CBD231、CBD279同源性为72%，丙型肝炎患者及健康献血员感染率最高。结论：在＊慢性乙型肝炎患者体内存在TLMV感染。
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| 关 键 词： | 输血传播病毒样微小病毒 序列分析 DNA |
| 文章编号： | 1671-587X(2006)06-1045-03 |
| 收稿时间： | 2006-04-03 |
| 修稿时间： | 2006年4月3日 |
Gene determination of transfusion transmitted virus-like mini virus |
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| LIU Yuan-yuan,MA Zhen-hua,WANG Feng,NIU Jun-qi,YU Yong-li.Gene determination of transfusion transmitted virus-like mini virus[J].Journal of Jilin University: Med Ed,2006,32(6):1045-1047. |
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| Authors: | LIU Yuan-yuan MA Zhen-hua WANG Feng NIU Jun-qi YU Yong-li |
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| Institution: | 1.Department of Infectious Diseases,First Hospital,Jilin University,Changchun 130021, China; 2.Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China |
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| Abstract: | Objective To detect the transfusion transmitted virus-like mini virus(TLMV) sequence gene in patients with chronic hepatitis and to study the TLMV infection in population.Methods Two sets of primers in the most conservative regions of Japan strains TLMV-CBD279 and CBD231 were designed to amplify TLMV templete extracted from sera of patients with chronic hepatitis B using PCR.PCR products were cloned into pGEMR-T vector and sequenced.Results The results showed that 72%of TLMV sequence isolated in China was identical to that in Japan,suggesting that there was TLMV infection in patients with chronic hepatitis B in China.The patients with hepatitis virus C and the healthy blood donor had the highest infectious rates of TLMV.Conclusion TLMV infection exists in patients with chronic hepatitis B in China. |
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| Keywords: | Transfusion transmitted virus TTV-like mini virus sequence DNA |
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