吡柔比星调控原钙黏蛋白10的表达对多发性骨髓瘤细胞增殖、凋亡诱导的作用机制研究

目的探讨吡柔比星(THP)对多发性骨髓瘤细胞增殖、凋亡的影响及其作用机制。方法 2014年4月-2019年3月在郴州市第一人民医院实验室进行实验。应用终浓度为0.1mg/L、1mg/L、2.5mg/L、5 mg/L吡柔比星处理多发性骨髓瘤细胞KM3,记为THP 0.1mg/L组、THP 1 mg/L组、THP 2.5mg/L组、THP 5mg/L组;未经任何处理的KM3细胞作空白对照(Con)组;转染pcDNA3.1为pcDNA3.1组,转染pcDNA3.1-PCDH10为pcDNA3.1-PCDH10组,转染si-NC后用1 mg/L的THP处理为THP 1 mg/L+si-NC组,转染si-PCDH10后用1 mg/L的THP处理为THP1 mg/L+si-PCDH10组。MTT法检测细胞活性,流式细胞术检测细胞凋亡,Western-blot法检测蛋白表达。结果不同浓度THP处理后,KM3细胞活性显著降低,细胞凋亡率显著升高,Cyclin D1、Bcl-2蛋白的表达水平显著降低,p21、Bax、PCDH10蛋白的表达水平显著升高(P <0.05);过表达PCDH10后,KM3细胞活性显著降低,细胞凋亡率显著升高,Cyclin D1、Bcl-2蛋白的表达水平显著降低,p21、Bax蛋白表达水平显著升高(P<0.05);抑制PCDH10表达后,THP处理的KM3细胞活性显著升高,细胞凋亡率显著降低,Cyclin D1、Bcl-2蛋白表达水平显著升高,p21、Bax蛋白的表达水平显著降低(P<0.05)。结论吡柔比星可抑制多发性骨髓瘤细胞的增殖,促进其凋亡,其机制可能与调控PCDH10表达相关。

The effect of pirarubicin on the proliferation and apoptosis of multiple myeloma cells

Objective To investigate the effect of THP on proliferation and apoptosis of multiple myeloma cells and its mechanism.Methods From April 2014 to March 2019,experiments were performed in the Laboratory of Chenzhou First People’s Hospital.Multiple myeloma cells KM3 were treated with a final concentration of 0.1 mg/L,1 mg/L,2.5 mg/L,5 mg/L pirarubicin,and recorded as THP 0.1 mg/L group,THP 1 mg/L group,THP 2.5 mg/L group,THP 5 mg/L group.Any treated KM3 cells were used as a blank control(Con) group.PcDNA3.1 was transfected into pcDNA3.1 group,pcDNA3.1 PCDH10 was transfected into pcDNA3.1 PCDH10 group,transfected with si NC and treated with 1 mg/L THP into THP 1 mg/L+si-NC group,transfected After treatment with si-PCDH10,1 mg/L of THP was used as the THP 1 mg/L+si-PCDH10 group.MTT assay was used to detect cell viability;flow cytometry was used to detect cell expression;Western blot was used to detect protein expression.Results After treatment with different concentrations of THP,the activity of KM3 cells decreased significantly,the apoptosis rate increased significantly,the expression level of cyclin D1 and Bcl-2 protein decreased significantly,and the expression level of p21,Bax and PCDH10 protein increased significantly(P <0.05).After overexpression of PCDH10,the activity of KM3 cells decreased significantly,the apoptosis rate increased significantly,the expression levels of cyclin D1 and Bel-2 protein decreased significantly,and the expression levels of p21 and Bax protein increased significantly(P <0.05).After inhibition of PCDH10 expression,the activity of KM3 cells treated with THP increased significantly,the apoptosis rate decreased significantly,the expression levels of cyclin D1 and Bcl-2 protein increased significantly,and the expression levels of p21 and Bax protein decreased significantly(P <0.05).Conclusion Pirarubicin can inhibit the proliferation and promote the apoptosis of multiple myeloma cells.Its mechanism may be related to the regulation of PCDH10 expression.