七氟烷对大鼠脑内腺苷酸环化酶及磷酸二酯酶活性的影响

目的观察七氟烷麻醉后对大鼠不同脑区、不同时期腺苷酸环化酶及磷酸二酯酶活性的影响,探讨3’,5’-环腺苷酸（cAMP）在七氟烷麻醉中的作用。方法SD大鼠40只,分层随机区组设计分成5组,每组8只,吸入1.5%的七氟烷,分别在未吸入七氟烷时（对照组）、翻正反射即将消失时（诱导期组）、翻正反射消失后2min（麻醉期组）、翻正反射恢复即刻（恢复期组）和翻正反射恢复后1h（清醒期组）断头取脑。放射免疫法测定大脑皮质、海马和脑干的腺苷酸环化酶（AC）活性及磷酸二酯酶（PDE）活性。结果（1）在对AC活性影响上,与对照组比较,诱导期组、麻醉期组的皮质、海马、脑干及恢复期组皮质、海马AC活性升高;与麻醉期组比较,清醒期组皮质、海马、脑干AC活性降低。上述差异均有统计学意义（均P〈0.05）。（2）在对PDE活性的影响上,与对照组比较,诱导期组、麻醉期组皮质、海马、脑干PDE活性下降;与麻醉期组比较,恢复期组、清醒期组皮质、海马、脑干PDE活性升高。上述差异均有统计学意义（均P〈0.05）。结论cAMP在七氟烷的全麻机制中可能发挥重要作用。

Effects of Sevoflurane on Adenylate Cyclase and Phosphodiesterases Activity in Brain of Rats

Objective To investigate the effects of sevoflurane on c adenylate cyclase （AC） and phosphodiesterases（PDE） activity in the cerebrocortex ,hippocampus and brain stem of rats, and to exam- ine the role of cAMP in sevoflurane anesthesia. Methods Fourty SD rats were delaminately designed and allocated randomly to 5 groups inhaling 1.5% sevoflurane i.e., no recovery （recovery group,n=8） and one hour after righting reflexrecovery（aware group,n=8）. The brain tissues were rapidly dissected in- to cerebrocortex and hippocampus and brain stem .Then the adenylate cyelase and phosphodiesterases activity were assessed. Results So far as the activity of AC is concerned, compared with the control group, the activity of AC in the cerebrocortex, hippocampus and brain stem brain stem of induction group and anesthesia group, the cerebrocortex, and hippocampus in the recovery group were significantly increased; compared with those in the anesthesia group, the activity of AC in the cerebrocortex, hip- pocampus and brain stem of aware group were significantly decreased （P〈0.05）; For the activity of PDE, compared with the control group, the activity of PDE in the cerebrocortex, hippocampus and brain stem in the induction group and anesthesia group was significantly decreased, compared with that in anesthe- sia group, the activity of PDE in the cerebrocortex , hippocampus and brain stem of recovery group and aware group was significantly increased （P〈0.05）. Conclusion cAMP may play an important role in sevoflurane anesthesia.