CD44蛋白表达与大肠癌淋巴结转移相关性研究

应用ＣＤ４４单抗对５８例石蜡包埋组织（３８例大脾性癌组织、９例癌旁组织和１１例淋巴结组织）进行免疫组化染色并进行图像分析定量研究。结果显示：３８例大肠癌，全呈ＣＤ４４性阳性反应，９例癌旁粘膜上皮细胞弱阳性，５例有癌转移的淋巴结呈阳性，６例无癌转移的淋巴结阴性。大肠癌化癌旁正常粘膜的染色强度要深，两者差异有极显著意义（Ｐ〈０．０１）。ＣＤ４４免疫反应强度与淋巴结与无转移有关，有淋巴结转移较无淋巴结转

The relation between CD 44 expression and lymph node metastasis of colorectal carcinoma

paraffin embedded colorectal carcinoma and 9 matched normal colon mucosa and 11 lymph nodes were tested by immunohistochemistry for CD 44 expression using CD 44 monoclonal antibody and immunohistochemical quantitative analysis using image analyser. The results showed that all colorectal carcinomas were positive and 9 nomal colon mucosa were weakly positive. The five metastatic lymph nodes were positive and the six non metastatis lymph nodes were negative. Statistical analysis revealed that the expression intensity of CD 44 was stronger in colorectal carcinomas than in normal colon mucosa ( P <0.01). The expression of CD 44 was more intense in metastatic (Dukes C/D) than in non metastatic (Dukes A/B) colorectal carcinomas ( P <0.01). Our results indicate that the abnomal expression of CD 44 is not only involved in the development, but also involoved in metastasis of colorectal carcinomas. Therefore, detection of CD 44 expression may have significant clinical importance in evaluation of the tendency of lymphatic metastasis.