褪黑素对卵巢癌SKOV3细胞侵袭、迁移和血管生成的影响

研究褪黑素对卵巢癌SKOV3细胞侵袭、迁移和血管生成的影响。MTT实验、划痕实验和Transwell侵袭实验分别观察褪黑素对SKOV3细胞增殖、侵袭和迁移的影响。诱导HUVECs成管,观察褪黑素对其影响。Western blot法检测SKOV3细胞内血管内皮生长因子(VEGF)、钙黏附蛋白E(E-cadherin)、基质金属蛋白酶9(MMP9)和波形蛋白(Vimentin)的表达。建立卵巢癌移植瘤模型,免疫组化检测CD31的表达。结果显示,褪黑素能抑制卵巢癌SKOV3细胞的侵袭和迁移,并且明显下调MMP-9、Vimentin和VEGF蛋白的表达,上调E-cadherin的表达。此外,褪黑素抑制了HUVECs的小管生成,体内CD31的免疫组化结果也显示褪黑素能够抑制微血管密度。结果提示,褪黑素能抑制卵巢癌细胞的侵袭、迁移和血管生成。

Effect of melatonin on invasion, migration and angiogenesis of ovarian cancer SKOV3 cells

To evaluate the effects of melatonin on invasion, migration and angiogenesis of ovarian cancer SKOV3 cells. The effects of melatonin on proliferation, invasion and migration of SKOV3 cells were observed by MTT assay, scratch assay and transwell invasion experiment. Then, the tube formation was observed after treating the HUVECs with melatonin. Western blot was used to detect the expression of vascular endothelial growth factor (VEGF) E-cadherin, matrix metalloprotein-9(MMP-9) and Vimentin protein in SKOV3 cells. The HUVECs was prepared to study effect of melatonin on tube formation ability. In addition, the expression of CD31 was observed in vivo SKOV3 xenograft model. The results showed that melatonin could inhibit the invasion and migration of SKOV3 cells, and down-regulate the expression of proteins such as MMP-9, Vimentin and VEGF, while the expression of E-cadherin increased. Furthermore, melatonin inhibited the tube formation of HUVECs, and the immunohistochemical results of CD31 also showed that melatonin can inhibit microvessel density in vivo. The results suggested that melatonin may inhibit the invasion, migration and angiogenesis of ovarian cancer cells.