苯并[a]芘对宫颈癌HeLa细胞细胞色素P450 1A1的诱导作用

目的探讨苯并a]芘（BaP）对宫颈癌HeLa细胞增殖及细胞色素P450 1A1（CYP1A1）表达的影响。方法不同浓度（0、1.0、2.5、5.0、7.5和10.0μmol/L）的BaP处理宫颈癌HeLa细胞,MTT法检测BaP对HeLa细胞增殖的影响,W estern b lotting和RT-PCR分别检测CYP1A1的蛋白表达和mRNA表达,荧光素标记检测CYP1A1活性。结果 MTT法检测显示,各浓度BaP组光密度（D553 nm）均较对照组（0μmol/L BaP）升高,其中7.5μmol/L BaP组最高（P〈0.05）。W estern b lotting和RT-PCR检测结果显示,各浓度BaP组CYP1A1 mRNA及蛋白的表达水平均高于对照组,其中7.5μmol/L BaP组最高（P〈0.05）。CYP1A1活性检测结果显示,各浓度BaP组HeLa细胞CYP1A1酶活力均高于对照组,以5.0μmol/L BaP组活力最高（P〈0.05）。结论 BaP可诱导HeLa细胞增殖,诱导HeLa细胞表达CYP1A1,并增加酶活性。 更多还原

Effects of benzo(a)pyrene on expression of cytochrome P450 1A1 in HeLa cells of uterine cervix cancer

Objective To investigate the effects of benzo(a)pyrene (BaP) on the proliferation of HeLa cells of uterine cervix cancer and expression of cytochrome P450 1A1 (CYP1A1) in HeLa cells of uterine cervix cancer. Methods HeLa cells of uterine cervix cancer were treated with different concentrations of BaP (0, 1.0, 2.5, 5.0, 7.5 and 10.0 μmol/L). The effect of BaP on the proliferation of HeLa cells was detected by MTT assay, the expression of CYP1A1 protein and mRNA was determined by Western blotting and RT-PCR, respectively, and the activity of CYP1A1 was measured by fluorescein labeling. Results MTT assay revealed that optical density (D553 nm) of groups of different concentrations of Bap was higher than that of control group (0 μmol/L BaP), with the highest value in 7.5 μmol/L BaP group (P<0.05). RT-PCR and Western blotting indicated that the expression of CYP1A1 mRNA and protein of groups of different concentrations of Bap was higher than that in control group, with the highest value in 7.5 μmol/L BaP group (P<0.05). The activity of CYP1A1 in groups of different concentrations of BaP was higher than that in control group, with the highest value in 5.0 μmol/L BaP group (P<0.05). Conclusion BaP can promote the proliferation of HeLa cells, and increase the expression and activity of CYP1A1 in HeLa cells.