丙酸氟替卡松对体外培养鼻黏膜上皮细胞和体内鼻息肉组织中水通道蛋白5表达的影响

目的:通过丙酸氟替卡松(FP)对体外原代培养的鼻黏膜上皮细胞和体内鼻息肉组织中水通道蛋白5(AQP5)表达的影响,初步探讨AQP5在鼻息肉发病中的可能机制.方法:将人鼻黏膜上皮细胞进行体外原代细胞培养14天,传代后FP组细胞加入丙酸氟替卡松浓度为0.2%的完全培养液,非FP组细胞加入不含丙酸氟替卡松的培养液继续培养,7天后爬片固定,采用免疫组化技术检测细胞中AQP5的表达.并测定阳性细胞的光密度值和灰度值.将12例慢性鼻窦炎鼻息肉患者(均为初发型),3个月内无局部及全身激素使用史,取其鼻息肉组织,术前应用FP喷雾剂喷鼻7天,治疗7天后术中再次取其鼻息肉组织,术后病理均证实为鼻息肉组织,采用免疫组化技术检测鼻息肉组织中AQP5的表达和分布.并测定阳性细胞的光密度值和灰度值.结果:在体外培养的上皮细胞FP组的AQP5表达的灰度值明显降低(P<0.05),光度值则升高(P<0.05),在体内鼻息肉组织中,患者使用FP后鼻息肉体积明显缩小,,用药后AQP5在腺体细胞和淋巴细胞中表达的灰度值明显升高(P<0.05),光密度值则明显低于使用前(P<0.05).结论:在FP作用下培养的鼻黏膜上皮细胞中的AQP5表达增加,可能解释在体情况下有利于组织间液的排除,减轻中鼻甲和下鼻甲的水肿,而在鼻息肉组织中,应用FP后,鼻息肉体积缩小,水肿减轻,可能与AQP5在腺体和淋巴细胞中表达减少有关,说明AQP5在鼻息肉的形成和发展过程中起到一定的作用.

The effects of intranasal FP on the expression of AQP5 in epithelial cells of nasal mucosa and nasal polyps

Objective:To study the effects of intranasal FP on the expression of AQP5 in epithelial cells of nasal mucosa and nasal polyps and further explore the possible mechanism of AQP5 in the onset of nasal polys. Methods:The primary culture of the epithelial cells of nasal mucosa was conducted for 14 days. After these cells were subcultured.The cells of FP group was treated with culture medium containing 0.2% FP.The cells of no FP group were treated with culture medium which was not containing FP. After 7 days,the expressions of AQP5 were studied in the cells with immunohistochemistric staining,by its integrated light intensity and its grey step. At the first day we obtain 12 nasal polyps samples from these inpatients who were trouble with chronic sinusitis and nasal polyps for the first time and were not treated with glucocorticoid partly or integreatly in the last three mouths. FP were intranasally used 7 days before operation.The other 12 nasal polyps were obtained and studied with immunohistochemical technique,by its light intensity and its grey step. Results:The grey step of AQP5 in the cells of FP-using group is lower obviously than using FP before(P < 0.05),the light intensity is higher obviously than using FP before(P < 0.05). In the nasal polyps of FP-using group,the size of nasal polyps become much smaller than using FP before.The grey step of AQP5 in both glandular cells and lymphocyte is higher obviously than using FP before(P < 0.05) but the light intensity is lower obviously than using FP before(P < 0.05). Conclusion:the expressions of AQP5 in cells which treated with FP became higher than cells which untreated,It indicated that when treated with FP,interstitial fluid can effuse easily,the concha nasalis media and the concha nasalis inferior became smaller and smaller. After nasal polyps samples treated with FP,the poloyps became smaller.At the same time,the expressions of AQP5 in glandular cells and lymphocyte became lower. There may be relationship between these. It can indicate that AQP5 contributed to edema of nasal polyps.