旋毛虫感染鼠血清筛选日本血吸虫成虫cDNA文库

目的 探讨旋毛虫与血吸虫之间交叉免疫的分子机制，并寻找有效的日本血吸虫疫苗侯选分子。方法 以旋毛虫感染鼠血清为探针，筛选日本血吸虫成虫cDNA文库，对获得的阳性克隆插入片段进行PCR扩增。结果 共筛选出11个阳性克隆，其插入的cDNA片段大小在1．4－5．0kb之间，其中1．4kb片段2个，5．0kb片段9个。结论 获得的阳性在隆插入基因片段可能为编码旋毛虫与日本血吸虫共同的抗原基因，其表达产物可能有抗日本血吸虫感染的作用。

IMMUNOSCREENING OF SCHISTOSOMA JAPONICUM ADULT WORM cDNA LIBRARY WITH SERA OF MICE INFECTED WITH TRICHINELLA SPIRALIS

ObjectivesTo explore the molecular mechanism of cross reactivity between Trichinella spiralis (Ts)andSchistosoma japonicum (Sj) , and to find out effective vaccine candidate antigens against schistosomiasis japonica. Methods Sj adult worm cDNA library was screened using sera of mice infected with Tsand the inserts of positive clones were specifically amplified by PCR. Results Eleven positive clones were obtained after three rounds of immunoscreening. The size of these inserts ranged from 1 4 to 5 0 kb. Two of these fragments were 1 4 kb, and nine were 5 0 kb. Conclusions The positive clones may be the genes encoding the same antigens ofTs and Sj, and their expressed products could be used as vaccine candidates forSj infection.