STZ诱导糖尿病肾病大鼠模型的建立

目的建立糖尿病大鼠动物模型,探讨其肾脏损害规律。方法用STZ65mg/kg一次性腹腔内注射方式制作糖尿病大鼠模型,设立空白对照组,饲养14周,期间观察大鼠血糖、尿糖及一般情况变化,实验结束时测定血肌酐、尿素氮、尿蛋白、尿白蛋白排泄率,取肾作病理及超微病理检查。结果模型组大鼠出现血肌酐、尿素氮、尿蛋白、尿白蛋白明显升高,出现肾脏肥大,病理显示明显的肾小球、肾小管病变。结论STZ诱导糖尿病大鼠肾脏表现肾小球及小管间质损害,可以用作糖尿病肾病研究的动物模型。

Establishment of STZ-Induced Diabetic Nephropathic Rat Model

Objective To establish a diabetic nephropathic rat model and to study it''s kidney lesions. Methods 40 male Wistar rats were randomly divided into control and experimental diabetic groups, 20 per each group. Diabetic rats were induced by one intraperitoneal injection of streptozotocin (STZ) in a dose of 65 mg/kg. The rats were breed for 14 weeks. During the experiment, the blood glucose and urine glucose were measured and gross health status was observed. Finally, serum creatinine (SCr), blood urea nitrogen (BUN), urine protein and urine albumin were assayed, and renal pathological changes were examined by light and electron microscopy. Results The diabetic rats showed increased SCr, BUN, urine protein and urine albumin levels and hypertrophy of the kidneys. The renal pathology exhibited glomerular and tubulo-interstitial alterations. Conclusion The STZ-induced rat diabetic model have certain glomerulus and tubulo-interstitial lesions and can be used in diabetic nephropathy studies.